Product Info Summary
SKU: | A01065 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™
SKU/Catalog Number
A01065
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ catalog # A01065. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01065)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MUT, different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A01065 is reactive to MMUT in Human, Mouse, Rat
Applications
A01065 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
83 kDa
Calculated molecular weight
83.134kDa
Background of MMUT
Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MUT using anti-MUT antibody (A01065).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: human K562 whole cell lysates,
lane 2: huam CACO-2 whole cell lysates,
lane 3: rat liver tissue lysates,
lane 4: rat kidney tissue lysates,
lane 5: rat heart tissue lysates,
lane 6: mouse liver tissue lysates,
lane 7: mouse kidney tissue lysates,
lane 8: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUT antigen affinity purified polyclonal antibody (Catalog # A01065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUT at approximately 83KD. The expected band size for MUT is at 83KD.
Click image to see more details
Figure 2. IHC analysis of MUT using anti-MUT antibody (A01065).
MUT was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUT Antibody (A01065) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MUT using anti-MUT antibody (A01065).
MUT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUT Antibody (A01065) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of MUT using anti-MUT antibody (A01065).
MUT was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MUT Antibody (A01065) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of MUT using anti-MUT antibody (A01065).
MUT was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MUT Antibody (A01065) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of A431 cells using anti-MUT antibody (A01065).
Overlay histogram showing A431 cells stained with A01065 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MUT Antibody (A01065, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MMUT (Source: Uniprot.org, NCBI)
Gene Name
MMUT
Full Name
Methylmalonyl-CoA mutase, mitochondrial
Weight
83.134kDa
Superfamily
methylmalonyl-CoA mutase family
Alternative Names
Methylmalonyl-CoA mutase, mitochondrial MMUT MCM, MUT methylmalonyl-CoA mutase methylmalonyl-CoA mutase, mitochondrial|methylmalonyl Coenzyme A mutase|methylmalonyl-CoA isomerase|methylmalonyl-CoA mutase c.*192delA|methylmalonyl-CoA mutase c.*51C>G|methylmalonyl-CoA mutase variant c.1495G>A|methylmalonyl-CoA mutase variant c.2011A>G|methylmalonyl-CoA mutase variant c.2150G>T|methylmalonyl-CoA mutase variant c.322C>T|methylmalonyl-CoA mutase variant c.613_615delGAA|methylmalonyl-CoA mutase variant c.636G>A|methylmalonyl-CoA mutase variant c.643G>A|mutant methylmalonyl CoA mutase|truncated methylmalonyl CoA mutase|truncated methylmalonyl-CoA mutase variant c.1420C>T|truncated methylmalonyl-CoA mutase variant c.2179C>T|truncated methylmalonyl-CoA mutase variant c.91C>T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMUT, check out the MMUT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMUT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ (A01065)
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No publications found for A01065
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
4 Customer Q&As for Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™
Question
Can A01065 be used for flow cytometry? Do you have the tested data?
Verified customer
Asked: 2021-11-11
Answer
The Anti-Methylmalonyl Coenzyme A Mutase Antibody Picoband™ (A01065) can be used for flow cytometry and here is the tested data: https://www.bosterbio.com/anti-mut-picoband-trade-antibody-a01065-boster.html
Boster Scientific Support
Answered: 2021-11-16
Question
We are currently using anti-Methylmalonyl Coenzyme A mutase antibody A01065 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The anti-Methylmalonyl Coenzyme A mutase antibody (A01065) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-18
Question
Would A01065 anti-Methylmalonyl Coenzyme A mutase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
You can see on the product datasheet, A01065 anti-Methylmalonyl Coenzyme A mutase antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-08
Question
Is a blocking peptide available for product anti-Methylmalonyl Coenzyme A mutase antibody (A01065)?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
We do provide the blocking peptide for product anti-Methylmalonyl Coenzyme A mutase antibody (A01065). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-24