Anti-MyD88 Monoclonal Antibody [2E9C2]

Product Name

Anti-MyD88 Monoclonal Antibody [2E9C2]

View all MYD88 Antibodies

SKU/Catalog Number

M00025-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-MyD88 Monoclonal Antibody [2E9C2] (Catalog # M00025-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

MyD88 monoclonal antibody can be stored at -20°C, stable for one year.

Cite This Product

Anti-MyD88 Monoclonal Antibody [2E9C2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00025-2)

Host

Mouse

Contents

MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

Clone: 2E9C2

Isotype

IgG2b

Immunogen

Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.

Reactive Species

M00025-2 is reactive to MYD88 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.2 kDa

Background of MYD88

The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00025-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information MYD88

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