Product Info Summary
SKU: | M00025-2 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-MyD88 Monoclonal Antibody [2E9C2]
SKU/Catalog Number
M00025-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MyD88 Monoclonal Antibody [2E9C2] (Catalog # M00025-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
MyD88 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-MyD88 Monoclonal Antibody [2E9C2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00025-2)
Host
Mouse
Contents
MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 2E9C2
Isotype
IgG2b
Immunogen
Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.
Reactive Species
M00025-2 is reactive to MYD88 in Human, Mouse
Applications
M00025-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.233kDa
Background of MYD88
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MyD88 in EL4 whole cell lysate with MyD88 antibody at 2 μg/mL.
Click image to see more details
Immunohistochemistry of MyD88 in human heart with MyD88 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MYD88 (Source: Uniprot.org, NCBI)
Gene Name
MYD88
Full Name
Myeloid differentiation primary response protein MyD88
Weight
33.233kDa
Alternative Names
MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88 MYD88 IMD68D, MYD88 MYD88 innate immune signal transduction adaptor myeloid differentiation primary response protein MyD88|mutant myeloid differentiation primary response 88|myeloid differentiation primary response 88|myeloid differentiation primary response gene (88)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYD88, check out the MYD88 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYD88: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MyD88 Monoclonal Antibody [2E9C2] (M00025-2)
Hello CJ!
M00025-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X,Shi MQ,Chen C,Du JR.Phthalide derivative CD21 ameliorates ischemic brain injury in a mouse model of global cerebral ischemia: involvement of inhibition of NLRP3.Int Immunopharmacol.2020 Sep;86:106714.doi: 10.1016/j.intimp.2020.106714.Epub 2020 Jun 24
Species: Mouse
M00025-2 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:250
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