Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | M03915 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MYO7A Monoclonal Antibody
SKU/Catalog Number
M03915
BM5700 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MYO7A Monoclonal Antibody catalog # M03915. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MYO7A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03915)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFCH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myosin VIIa Myosins
Reactive Species
M03915 is reactive to MYO7A in Human, Mouse, Rat
Observed Molecular Weight
254 kDa
Calculated molecular weight
254.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03915 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:100
Positive Control
WB: Y79 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Myosin VIIa expression in Y79 cell lysate.
Click image to see more details
IHC analysis of MYO7A using anti-MYO7A antibody (M03915).
MYO7A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MYO7A using anti-MYO7A antibody (M03915).
MYO7A was detected in a paraffin-embedded section of mouse eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MYO7A using anti-MYO7A antibody (M03915).
MYO7A was detected in a paraffin-embedded section of rat eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of MYO7A using anti-MYO7A antibody (M03915).
MYO7A was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-MYO7A Antibody (M03915) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-MYO7A Monoclonal Antibody (M03915)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MYO7A Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-MYO7A Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-MYO7A Monoclonal Antibody
Question
Is this M03915 anti-MYO7A Monoclonal antibody reactive to the isotypes of MYO7A?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The immunogen of M03915 anti-MYO7A Monoclonal antibody is A synthesized peptide derived from human Myosin VIIa Myosins. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-04
Question
Is a blocking peptide available for product anti-MYO7A Monoclonal antibody (M03915)?
N. Anderson
Verified customer
Asked: 2019-12-04
Answer
We do provide the blocking peptide for product anti-MYO7A Monoclonal antibody (M03915). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-04
Question
I have attached the WB image, lot number and protocol we used for retina using anti-MYO7A Monoclonal antibody M03915. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-21
Question
We are currently using anti-MYO7A Monoclonal antibody M03915 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The anti-MYO7A Monoclonal antibody (M03915) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-02
Question
I was wanting to use your anti-MYO7A Monoclonal antibody for WB for human retina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human retina identification?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
As indicated on the product datasheet, M03915 anti-MYO7A Monoclonal antibody has been tested for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08
Question
Do you have a BSA free version of anti-MYO7A Monoclonal antibody M03915 available?
T. Kulkarni
Verified customer
Asked: 2014-11-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MYO7A Monoclonal antibody M03915 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-11-10
