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Product Info Summary
| SKU: | A08502 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Myosin-8 MYH8 Antibody
SKU/Catalog Number
A08502
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Myosin-8 MYH8 Antibody (Catalog # A08502). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MYH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Myosin-8 MYH8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08502)
Host
Rabbit
Contents
MYH8 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8. The immunogen is located within amino acids 60 - 110 of MYH8.
Cross-reactivity
MYH8 antibody is human, mouse and rat reactive. MYH8 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Reactive Species
A08502 is reactive to MYH8 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
222.8 kDa
Background of MYH8
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08502 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MYH8 in HeLa cell lysate with MYH8 antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunohistochemistry of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 5 μg/ml.
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Immunofluorescence of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 20 μg/ml.
Specific Publications For Anti-Myosin-8 MYH8 Antibody (A08502)
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