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- Table of Contents
Nox4 antibody (PA1929) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IHC analysis of NOX4 using anti-NOX4 antibody (PA1929).
NOX4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX4 Antibody (PA1929) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NOX4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX4 Antibody (PA1929) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NADPH oxidase 4/NOX4 Antibody Picoband®
Nox4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
Nox4 antibody was positively detected by WB in:
human BEAS-2B whole cell, human U2OS whole cell, human SH-SY5Y whole cell, human Hela whole cell, human HepG2 whole cell, human PC-3 whole cell, human A549 whole cell, rat heart tissue, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Nox4 antibody was positively detected by IHC in:
human kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Nox4 antibody was positively detected by FC in:
Beas-2B cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NADPH oxidase 4/NOX4 Antibody Picoband®
View all NOX4 Antibodies
View all NOX4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4, identical to the related rat sequence and different from the related human sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated Molecular Weight
66.5 kDa
Background of Nox4
NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-NADPH oxidase 4/NOX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1929)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
100%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Human
88%
Moderate Homology
Monkey
88%
Moderate Homology
Rabbit
81%
Moderate Homology
Zebrafish
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Nox4 antibody
Immunohistochemistry (IHC) protocol for Nox4 antibody
Flow Cytometry (FC) protocol for Nox4 antibody
Publications
Application:
Species:
PM2.5-induced oxidative stress upregulates PLA2R expression in the lung and is involved in the pathogenesis of membranous nephropathy through extracellular vesicles
Published: 2024-12-18
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 39744137
THRA1/PGC-1α/SIRT3 pathway regulates oxidative stress and is implicated in hypertension of maternal hypothyroid rat offspring
Published: 2024-12-06
Journal: HYPERTENSION RESEARCH
Application: WB
Impact Factor: 4.30
Species: Rat
PMID: 39639130
In utero chronic intermittent nicotine aerosol exposure increases ischemic heart injury in adult offspring via programming of Angiotensin II receptor-derived TGFβ/ROS/Akt signaling pathway
Published: 2024-06-28
Journal: REPRODUCTIVE TOXICOLOGY
Application: WB
Impact Factor: 3.30
Species: Rat
PMID: 38945500
The CXCR4-AT1 axis plays a vital role in glomerular injury via mediating the crosstalk between podocyte and mesangial cell
Published: 2023-09-09
Journal: Translational Research
Application: IHC,WB
Impact Factor: 7.80
Species: Mouse
PMID: 37696390
Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects
Published: 2023-02-05
Journal: Biomedicines
Application: WB,IHC
Impact Factor: 4.70
Species: Mouse,Human
PMID: 36830999
Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress
Published: 2022-08-12
Journal: REPRODUCTIVE TOXICOLOGY
Application: WB
Impact Factor: 3.42
Species: Rat
PMID: 35970333
Renal and cerebral RAS interaction contributes to diabetic kidney disease
Published: 2019-05-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.27
Species: Rat
PMID: 31217864
Epigenetic Down-Regulation of Sirt 1 via DNA Methylation and Oxidative Stress Signaling Contributes to the Gestational Diabetes Mellitus-Induced Fetal Programming of Heart Ischemia-Sensitive Phenotype in Late Life
Published: 2019-05-11
Journal: International Journal of Biological Sciences
Application: WB
Impact Factor: 4.07
Species: Rat
PMID: 31223283
C-X-C Chemokine Receptor Type 4 Plays a Crucial Role in Mediating Oxidative Stress-Induced Podocyte Injury
Published: 2017-08-20
Journal: ANTIOXIDANTS & REDOX SIGNALING
Application: WB
Impact Factor: 6.34
Species: Mouse
PMID: 27960539
Inhibition of CPU0213, a Dual Endothelin Receptor Antagonist, on Apoptosis via Nox4-Dependent ROS in HK-2 Cells
Published: 2016-06-24
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 4.65
Species: Human
PMID: 27336467
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Product Promise
We promise Anti-NADPH oxidase 4/NOX4 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1929
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026