|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-NADPH Oxidase 4 Antibody|
|Description||Rabbit IgG polyclonal antibody for NADPH oxidase 4(NOX4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-NADPH Oxidase 4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1929)|
|Replacement Item||This antibody may replace the following items: sc-21860|sc-55142|sc-30141 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||NADPH oxidase 4|
|Molecular Weight||66519 MW|
|Protein Function||Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. .|
|Tissue Specificity||EXpressed in brain, in all layers of the cerebellum, in pyramidal cells of the Ammon horn and in Purkinje cells (at protein level). Expressed in osteoclasts, leukocytes, kidney, liver and lung. .|
|Sequence Similarities||Contains 1 FAD-binding FR-type domain.|
|Subcellular Localization||Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell junction, focal adhesion . Cell membrane . May localize to plasma membrane and focal adhesions. .|
|Alternative Names||NADPH oxidase 4;1.6.3.-;Kidney oxidase-1;KOX-1;Kidney superoxide-producing NADPH oxidase;Renal NAD(P)H-oxidase;Superoxide-generating NADPH oxidase 4;Nox4;Renox;|
Background for NADPH oxidase 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NADPH Oxidase 4 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NADPH Oxidase 4 Antibody Images
Click the images to enlarge.
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Cardiac Muscle Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: SMMC Cell Lysate
IHC(P): Rat Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,