Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody

Product Name

Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody

View all NLRP3/NALP3 Antibodies

SKU/Catalog Number

M00034

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody catalog # M00034. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NALP3 NLRP3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00034)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FBD-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NALP3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00034 is reactive to NLRP3 in Human

Applications

M00034 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

118.173kDa

Background of NLRP3/NALP3

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.

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Western blot analysis of NLRP3 expression in SH-SY5Y cell lysate.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using NALP3 Antibody.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Immunofluorescent analysis of Raw264.7 cells, using NALP3 Antibody.

Gene/Protein Information For NLRP3 (Source: Uniprot.org, NCBI)

Alternative Names

AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; mouse NLRP3; NLRP3; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3 mouse; NLRP3 polyclonal; NLRP3 rat; NLRP3; Nucleotide-Binding Oligomerization Domain, Le NLRP3 AGTAVPRL, AII, AVP, C1orf7, CIAS1, CLR1.1, DFNA34, FCAS, FCAS1, FCU, KEFH, MWS, NALP3, PYPAF1 NLR family pyrin domain containing 3 NACHT, LRR and PYD domains-containing protein 3|NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3|NACHT, LRR and PYD containing protein 3|PYRIN-containing APAF1-like protein 1|caterpiller protein 1.1|cold autoinflammatory syndrome 1 protein|cold-induced autoinflammatory syndrome 1 protein|cryopyrin|cryopyrin, NACHT, LRR and PYD domains - containing protein 3|deafness, autosomal dominant 34|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3

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