Click image to see more details
Product Info Summary
| SKU: | M00153 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Nanog Rabbit Monoclonal Antibody
SKU/Catalog Number
M00153
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Nanog Rabbit Monoclonal Antibody catalog # M00153. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nanog Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00153)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EHD-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nanog
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00153 is reactive to Nanog in Human
Applications
M00153 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
119 kDa
Calculated molecular weight
34.24kDa
Background of Nanog
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Immunofluorescent analysis of NCCIT cells, using Nanog Antibody.
Click image to see more details
Western blot analysis of Nanog expression in NCCIT cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat testis, using Nanog Antibody.
Protein Target Info & Infographic
Gene/Protein Information For Nanog (Source: Uniprot.org, NCBI)
Gene Name
Nanog
Full Name
Homeobox protein NANOG
Weight
34.24kDa
Superfamily
Nanog homeobox family
Alternative Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog Nanog|2410002E02Rik, EN, ENK, ecat, ecat4|Nanog homeobox|homeobox protein NANOG|ES cell-associated protein 4|early embryo specific expression NK family|early embryo specific expression NK-type homeobox protein|homeobox transcription factor Nanog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nanog, check out the Nanog Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nanog: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nanog Rabbit Monoclonal Antibody (M00153)
Hello CJ!
No publications found for M00153
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Nanog Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Nanog Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Nanog Rabbit Monoclonal Antibody
Question
Would anti-Nanog Rabbit Monoclonal antibody M00153 work for IF with embryonic stem cell?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
According to the expression profile of embryonic stem cell, NANOG is highly expressed in embryonic stem cell. So, it is likely that anti-Nanog Rabbit Monoclonal antibody M00153 will work for IF with embryonic stem cell.
Boster Scientific Support
Answered: 2020-03-31
Question
We are currently using anti-Nanog Rabbit Monoclonal antibody M00153 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-Nanog Rabbit Monoclonal antibody (M00153) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
Is there a BSA free version of anti-Nanog Rabbit Monoclonal antibody M00153 available?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Nanog Rabbit Monoclonal antibody M00153 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-25
Question
Is a blocking peptide available for product anti-Nanog Rabbit Monoclonal antibody (M00153)?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
We do provide the blocking peptide for product anti-Nanog Rabbit Monoclonal antibody (M00153). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-03
Question
I was wanting to use your anti-Nanog Rabbit Monoclonal antibody for IF for human embryonic stem cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryonic stem cell identification?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
It shows on the product datasheet, M00153 anti-Nanog Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic stem cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-15
Question
I would like to test anti-Nanog Rabbit Monoclonal antibody M00153 on human embryonic stem cell for research purposes, then I may be interested in using anti-Nanog Rabbit Monoclonal antibody M00153 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Krishna
Verified customer
Asked: 2019-07-15
Answer
The products we sell, including anti-Nanog Rabbit Monoclonal antibody M00153, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-15
Question
My question regarding product M00153, anti-Nanog Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00153 anti-Nanog Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-19
Question
Will M00153 anti-Nanog Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
You can see on the product datasheet, M00153 anti-Nanog Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-17
Question
We have seen staining in human cerebellar vermis. Are there any suggestions? Is anti-Nanog Rabbit Monoclonal antibody supposed to stain cerebellar vermis positively?
Verified Customer
Verified customer
Asked: 2018-09-11
Answer
Based on literature cerebellar vermis does express NANOG. Based on Uniprot.org, NANOG is expressed in cerebellar vermis, embryonic stem cell, teratocarcinoma, among other tissues. Regarding which tissues have NANOG expression, here are a few articles citing expression in various tissues:
Embryonic stem cell, Pubmed ID: 12787504, 14990856, 16391521
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-09-11
Question
Will anti-Nanog Rabbit Monoclonal antibody M00153 work on primate WB with teratocarcinoma?
G. Yang
Verified customer
Asked: 2018-01-19
Answer
Our lab technicians have not validated anti-Nanog Rabbit Monoclonal antibody M00153 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Nanog Rabbit Monoclonal antibody M00153 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate teratocarcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-19
Question
Please see the WB image, lot number and protocol we used for embryonic stem cell using anti-Nanog Rabbit Monoclonal antibody M00153. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic stem cell using anti-Nanog Rabbit Monoclonal antibody M00153. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-06
Question
I see that the anti-Nanog Rabbit Monoclonal antibody M00153 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-30
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-30
Question
Is this M00153 anti-Nanog Rabbit Monoclonal antibody reactive to the isotypes of NANOG?
Z. Zhao
Verified customer
Asked: 2017-03-28
Answer
The immunogen of M00153 anti-Nanog Rabbit Monoclonal antibody is A synthesized peptide derived from human Nanog. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-03-28
Question
We ordered your anti-Nanog Rabbit Monoclonal antibody for WB on embryonic stem cell a few years ago. I am using human, and We are going to use the antibody for IF next. you antibody examining embryonic stem cell as well as teratocarcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
J. Walker
Verified customer
Asked: 2014-09-22
Answer
I viewed the website and datasheets of our anti-Nanog Rabbit Monoclonal antibody and it seems that M00153 has been validated on human in both WB and IF. Thus M00153 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-09-22
Question
Our team were well pleased with the WB result of your anti-Nanog Rabbit Monoclonal antibody. However we have seen positive staining in embryonic stem cell nucleus using this antibody. Is that expected? Could you tell me where is NANOG supposed to be expressed?
J. Jones
Verified customer
Asked: 2014-02-05
Answer
From literature, embryonic stem cell does express NANOG. Generally NANOG expresses in nucleus. Regarding which tissues have NANOG expression, here are a few articles citing expression in various tissues:
Embryonic stem cell, Pubmed ID: 12787504, 14990856, 16391521
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-02-05
