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- Table of Contents
NANOS3 antibody (A07449) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of Nanos3 in Human Brain tissue with Nanos3 antibody at 20 μg/mL.
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Anti-Nanos homolog 3 Nanos3 Antibody
NANOS3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Nanos homolog 3 Nanos3 Antibody
View all NANOS3 Antibodies
View all NANOS3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Nanos3 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
Nanos3 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3. The immunogen is located within the last 50 amino acids of Nanos3.
Cross-Reactivity
This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
18.8 kDa
Background of NANOS3
Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.
Cite This Product
Anti-Nanos homolog 3 Nanos3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07449)
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Product Promise
We promise Anti-Nanos homolog 3 Nanos3 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A07449
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026