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Product Info Summary
| SKU: | A13562 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NAT11 NAA40 Antibody
SKU/Catalog Number
A13562
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NAT11 NAA40 Antibody (Catalog # A13562). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
NAT11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NAT11 NAA40 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13562)
Host
Rabbit
Contents
NAT11 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NAT11 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NAT11. The immunogen is located within the last 50 amino acids of NAT11.
Reactive Species
A13562 is reactive to NAA40 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
27.2 kDa
Background of NAA40
N-terminal acetylation is one of the most common protein modifications in eukaryotes, occurring on approximately 57% and 84% on yeast and human proteins respectively. There are several N-terminal acetylating enzyme complexes (NatA - NatE). Unlike the other complexes, NatD is composed of a single protein, NAT11, and has recently been described to acetylate the Serine N-termini of histones H2A and H4 in yeast. The role these modifications play is unknown; yeast that do not express NAT11 grow at normal rates and have no observable phenotypes. The role of the human homolog is likewise unknown.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13562 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NAT11 antibody can be used for detection of NAT11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NAT11 in human thymus tissue lysate with NAT11 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of NAT11 in mouse thymus tissue with NAT11 antibody at 5 μg/mL.
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Immunofluorescence of NAT11 in mouse thymus tissue with NAT11 antibody at 20 μg/mL.
Specific Publications For Anti-NAT11 NAA40 Antibody (A13562)
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