Product Info Summary
SKU: | M01337 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NCoA-3 Monoclonal Antibody
View all NCOA3/AIB1 Antibodies
SKU/Catalog Number
M01337
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NCoA-3 Monoclonal Antibody catalog # M01337. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NCoA-3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01337)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2C11B12
Isotype
IgG
Immunogen
Purified recombinant fragment of NCoA-3 (aa1-200) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01337 is reactive to NCOA3 in Human
Applications
M01337 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
155.293kDa
Background of NCOA3/AIB1
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-NCoA-3 Monoclonal Antibody M01337
Western Blot (WB) analysis using NCoA-3 Monoclonal Antibody against truncated Trx-NCoA-3 recombinant protein (1) and truncated NCoA-3 (aa1-200)-hIgGFc transfected CHOK1 cell lysate (2).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For NCOA3 (Source: Uniprot.org, NCBI)
Gene Name
NCOA3
Full Name
Nuclear receptor coactivator 3
Weight
155.293kDa
Superfamily
SRC/p160 nuclear receptor coactivator family
Alternative Names
ACTR; ACTRCBP-interacting protein; AIB1; AIB1AIB-1; Amplified in breast cancer 1 protein; BHLHE42; bHLHe42p/CIP; CAGH16; Class E basic helix-loop-helix protein 42; CTG26; KAT13B; MGC141848; NCOA3; NCoA-3; nuclear receptor coactivator 3; pCIP ; pCIP; RAC3; RAC-3; RAC3pCIP; Receptor-associated coactivator 3; SRC-1; SRC3; SRC-3; SRC-3EC 2.3.1.48; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC16; TRAM1; TRAM-1TNRC14 NCOA3 ACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP nuclear receptor coactivator 3 nuclear receptor coactivator 3|CBP-interacting protein|amplified in breast cancer 1 protein|class E basic helix-loop-helix protein 42|receptor-associated coactivator 3|steroid receptor coactivator protein 3|thyroid hormone receptor activator molecule 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCOA3, check out the NCOA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCOA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NCoA-3 Monoclonal Antibody (M01337)
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No publications found for M01337
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