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Product Info Summary
| SKU: | A05814-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-NCOA7 Antibody (N-term)
SKU/Catalog Number
A05814-2
Size
80 µl
Description
Boster Bio Anti-NCOA7 Antibody (N-term) (Catalog # A05814-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NCOA7 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05814-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
Reactive Species
A05814-2 is reactive to NCOA7 in Human, Mouse
Calculated molecular weight
106.2 kDa
Background of NCOA7
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05814-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-NCOA7 Antibody (N-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 106 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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NCOA7 Antibody (N-term) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-NCOA7 Antibody (N-term) (A05814-2)
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