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NCSTN antibody (PA2250) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of NCSTN using anti-NCSTN antibody (PA2250).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCSTN antigen affinity purified polyclonal antibody (Catalog # PA2250) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCSTN at approximately 120-130 kDa. The expected band size for NCSTN is at 78 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCSTN antigen affinity purified polyclonal antibody (Catalog # PA2250) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCSTN at approximately 120-130 kDa. The expected band size for NCSTN is at 78 kDa.
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Anti-Nicastrin/NCSTN Antibody Picoband®
NCSTN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
NCSTN antibody was positively detected by WB in:
human 293T whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Human
Tested
Bovine
Predicted
Dog
Predicted
Mouse
Predicted
Rabbit
Predicted
Sheep
Predicted
Monkey
Predicted
Product Information
Product Name
Anti-Nicastrin/NCSTN Antibody Picoband®
View all NCSTN Antibodies
View all NCSTN Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Nicastrin, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120-130 kDa
Calculated Molecular Weight
78.4 kDa
Background of NCSTN
Nicastrin, also known as NCSTN, is a protein that is a part of the gamma secretase protein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretase protein complex, all of which undergo significant post-translational modification before becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin has also been identified as a regulator of neprilysin, an enzyme involved in the degradation of amyloid beta fragment.
Cite This Product
Anti-Nicastrin/NCSTN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2250)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Dog
100%
High Homology
Mouse
94%
High Homology
Rabbit
94%
High Homology
Sheep
94%
High Homology
Monkey
94%
High Homology
Rat
88%
Moderate Homology
Zebrafish
53%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NCSTN antibody
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Product Promise
We promise Anti-Nicastrin/NCSTN Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2250
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026