Anti-NDUFA2 Antibody Picoband™

Product Name

Anti-NDUFA2 Antibody Picoband™

View all NDUFA2 Antibodies

SKU/Catalog Number

A10396-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NDUFA2 Antibody Picoband™ catalog # A10396-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NDUFA2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10396-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NDUFA2 recombinant protein (Position: S7-A99).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10396-1 is reactive to NDUFA2 in Human, Mouse

Applications

A10396-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

10.922kDa

Background of NDUFA2

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 is a protein that in humans is encoded by the NDUFA2 gene. The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of NDUFA2 using anti-NDUFA2 antibody (A10396-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA2 antigen affinity purified polyclonal antibody (Catalog # A10396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA2 at approximately 14 kDa. The expected band size for NDUFA2 is at 11 kDa.

Gene/Protein Information For NDUFA2 (Source: Uniprot.org, NCBI)

Alternative Names

B8; CD14; CIB8; CI-B8; complex I B8 subunit; Complex I-B8; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2 (8kD, B8); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; NADH-ubiquinone oxidoreductase B8 subunit; NADH-ubiquinone oxidoreductase subunit CI-B8 NDUFA2 B8, CD14, CIB8, MC1DN13 NADH:ubiquinone oxidoreductase subunit A2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2|NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa|NADH-ubiquinone oxidoreductase subunit CI-B8|complex I B8 subunit

For more info on NDUFA2, check out the NDUFA2 Infographic

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In this infographic, you will see the following information for NDUFA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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