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Product Info Summary
| SKU: | A05867 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NDUFS3 Antibody (C-term)
SKU/Catalog Number
A05867
Size
80 µl
Description
Boster Bio Anti-NDUFS3 Antibody (C-term) (Catalog # A05867). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NDUFS3 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05867)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NDUFS3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 251-284 amino acids from the C-terminal region of human NDUFS3.
Reactive Species
A05867 is reactive to NDUFS3 in Human, Mouse
Calculated molecular weight
30.2 kDa
Background of NDUFS3
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05867 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, HepG2, mouse NIH/3T3 cell line, human brain and mouse brain tissue lysate (from left to right), using NDUFS3 Antibody (C-term). A05867 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Western blot analysis of lysate from THP-1 cell line, using NDUFS3 Antibody (C-term) (Cat. #A05867). A05867 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Specific Publications For Anti-NDUFS3 Antibody (C-term) (A05867)
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