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Product Info Summary
| SKU: | A09729 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NEK3 Antibody
SKU/Catalog Number
A09729
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NEK3 Antibody catalog # A09729. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09729)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ATG4C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09729 is reactive to NEK3 in Human, Mouse, Rat
Applications
A09729 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57.705kDa
Background of NEK3
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation By similarity. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Chen J.M., Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-NEK3 Antibody A09729
WesternBlot (WB) analysis of NEK3 polyclonal antibodyElectrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For NEK3 (Source: Uniprot.org, NCBI)
Gene Name
NEK3
Full Name
Serine/threonine-protein kinase Nek3
Weight
57.705kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; glycogen synthase A kinase; HSPK 36; HSPK36; hydroxyalkyl-protein kinase; MGC29949; Never in mitosis A-related kinase 3; NIMA (never in mitosis gene a)-related kinase 3; NimA-related protein kinase 3; phosphorylase B kinase kinase; serine/threonine-protein kinase Nek3 NEK3 HSPK36 NIMA related kinase 3 serine/threonine-protein kinase Nek3|HSPK 36|NIMA (never in mitosis gene a)-related kinase 3|glycogen synthase A kinase|hydroxyalkyl-protein kinase|never in mitosis A-related kinase 3|nimA-related protein kinase 3|phosphorylase B kinase kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEK3, check out the NEK3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEK3 Antibody (A09729)
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No publications found for A09729
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Customer Reviews
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