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Product Info Summary
| SKU: | M02192 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nesprin 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02192
BM5589 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Nesprin 1 Rabbit Monoclonal Antibody catalog # M02192. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nesprin 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02192)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEFE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nesprin 1
Reactive Species
M02192 is reactive to SYNE1 in Human, Mouse, Rat
Observed Molecular Weight
131 kDa
Calculated molecular weight
1,011.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02192 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of SYNE1 using anti-SYNE1 antibody (M02192).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human PC12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNE1 antigen affinity purified monoclonal antibody (M02192) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYNE1 at approximately 131 kDa. The expected band size for SYNE1 is at 1011 kDa.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Specific Publications For Anti-Nesprin 1 Rabbit Monoclonal Antibody (M02192)
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Customer Q&As
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7 Customer Q&As for Anti-Nesprin 1 Rabbit Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02192?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Nesprin 1 expression in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
See attached the WB image, lot number and protocol we used for liver using anti-Nesprin 1 Monoclonal antibody M02192. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-23
Question
I see that the anti-Nesprin 1 Monoclonal antibody M02192 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-23
Question
My question regards to test anti-Nesprin 1 Monoclonal antibody M02192 on rat liver for research purposes, then I may be interested in using anti-Nesprin 1 Monoclonal antibody M02192 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The products we sell, including anti-Nesprin 1 Monoclonal antibody M02192, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-14
Question
Is a blocking peptide available for product anti-Nesprin 1 Monoclonal antibody (M02192)?
Verified Customer
Verified customer
Asked: 2018-12-12
Answer
We do provide the blocking peptide for product anti-Nesprin 1 Monoclonal antibody (M02192). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-12-12
Question
Will M02192 anti-Nesprin 1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Jones
Verified customer
Asked: 2017-02-09
Answer
As indicated on the product datasheet, M02192 anti-Nesprin 1 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-09
Question
We are currently using anti-Nesprin 1 Monoclonal antibody M02192 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
T. Banerjee
Verified customer
Asked: 2015-04-30
Answer
The anti-Nesprin 1 Monoclonal antibody (M02192) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-30
