Anti-Neurofilament Light NF-L Antibody

Product Name

Anti-Neurofilament Light NF-L Antibody

View all NF-L Antibodies

SKU/Catalog Number

M02482-3

Size

100ul

Description

Boster Bio Anti-Neurofilament Light NF-L Antibody catalog # M02482-3. Tested in IF, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neurofilament Light NF-L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02482-3)

Host

Rabbit

Contents

Supplied as an aliquot of serum plus 5mM NaN₃

Clonality

Polyclonal

Immunogen

Recombinant full length human NF-L protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02482-3 is reactive to NEFL in Bovine, Human, Mouse, Pig, Rat

Applications

M02482-3 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

61.517kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1: 10,000-1:15,000. IF/ICC: 1:5,000.

Validation Images & Assay Conditions

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Western blot analysis of different tissue lysates using rabbit pAb to NF-L, M02482-3, dilution 1:20,000. in green. [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at 68kDa corresponds to the NF-L protein.

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Immunofluorescent analysis of mouse cerebellum

Gene/Protein Information For NEFL (Source: Uniprot.org, NCBI)

Alternative Names

68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa NEFL CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110 neurofilament light neurofilament light polypeptide|light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|protein phosphatase 1, regulatory subunit 110

For more info on NEFL, check out the NEFL Infographic

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In this infographic, you will see the following information for NEFL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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