Anti-NF68 Nefl Antibody (Monoclonal, NR4)

NF-L antibody

Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Product Info Summary

SKU: MA1070
Size: 100 μg/vial
Reactive Species: Human, Mouse, Pig, Rat
Host: Mouse
Application: IHC, IHC-F, WB

Customers Who Bought This Also Bought

Product Name

Anti-NF68 Nefl Antibody (Monoclonal, NR4)

View all NF-L Antibodies

SKU/Catalog Number

MA1070

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NF68 Nefl Antibody (Monoclonal, NR4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1070)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

NR4

Isotype

Mouse IgG1

Immunogen

Pig spinal cord.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1070 is reactive to NEFL in Human, Mouse, Pig, Rat

Applications

MA1070 is guaranteed for IHC, IHC-F, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

61.517kDa

Background of NF-L

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat, By Heat
Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -
Western blot, 1-2μg/ml, Human, mouse, pig, rat

Validation Images & Assay Conditions

Gene/Protein Information For NEFL (Source: Uniprot.org, NCBI)

Gene Name

NEFL

Full Name

Neurofilament light polypeptide

Weight

61.517kDa

Superfamily

intermediate filament family

Alternative Names

68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa NEFL CMT1F, CMT2E, CMTDIG, NF-L, NF68, NFL, PPP1R110 neurofilament light neurofilament light polypeptide|light molecular weight neurofilament protein|neurofilament protein, light chain|neurofilament subunit NF-L|neurofilament triplet L protein|neurofilament, light polypeptide 68kDa|protein phosphatase 1, regulatory subunit 110

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NEFL, check out the NEFL Infographic

NEFL infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NEFL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for MA1070

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-NF68 Nefl Antibody (Monoclonal, NR4)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-NF68 Nefl Antibody (Monoclonal, NR4)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-NF68 Nefl Antibody (Monoclonal, NR4)

Question

We want to test anti-NF68 antibody (Monoclonal, NR4) MA1070 on rat fetal brain cortex for research purposes, then I may be interested in using anti-NF68 antibody (Monoclonal, NR4) MA1070 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

The products we sell, including anti-NF68 antibody (Monoclonal, NR4) MA1070, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-20

Question

We are currently using anti-NF68 antibody (Monoclonal, NR4) MA1070 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, pig, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

The anti-NF68 antibody (Monoclonal, NR4) (MA1070) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-14

Question

Can MA1070 be provided carrier free?

Verified customer

Asked: 2019-06-17

Answer

Unfortunately, we can't provide the Anti-NF68 Nefl Antibody (Monoclonal, NR4) (MA1070) in a carrier free format.

Boster Scientific Support

Answered: 2019-06-18

Question

I see that the anti-NF68 antibody (Monoclonal, NR4) MA1070 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-07

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain cortex using anti-NF68 antibody (Monoclonal, NR4) MA1070. Let me know if you need anything else.

D. Krishna

Verified customer

Asked: 2018-05-09

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-09

Question

I was wanting to use your anti-NF68 antibody (Monoclonal, NR4) for IHC for rat fetal brain cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain cortex identification?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

It shows on the product datasheet, MA1070 anti-NF68 antibody (Monoclonal, NR4) has been tested for IHC, WB on human, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain cortex in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-08

Question

Is a blocking peptide available for product anti-NF68 antibody (Monoclonal, NR4) (MA1070)?

B. Jha

Verified customer

Asked: 2017-08-18

Answer

We do provide the blocking peptide for product anti-NF68 antibody (Monoclonal, NR4) (MA1070). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-18

Order DetailsPrice
MA1070

100μg

$370
MA1070-10ug

10μg sample (liquid)

$99
MA1070-Biotin

100 μg Biotin conjugated

$570
MA1070-Cy3

100 μg Cy3 conjugated

$570
MA1070-Dylight488

100 μg Dylight488 conjugated

$570
MA1070-Dylight550

100 μg Dylight550 conjugated

$570
MA1070-Dylight594

100 μg Dylight594 conjugated

$570
MA1070-FITC

100 μg FITC conjugated

$570
MA1070-HRP

100 μg HRP conjugated

$570
MA1070-APC

100 μg APC conjugated

$670
MA1070-PE

100 μg PE conjugated

$670
MA1070-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
MA1070
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.