|Reactivity||Human, Pig, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-NF68 Antibody (Monoclonal, NR4)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for NF68, neurofilament, light polypeptide (NEFL ) detection. Tested with WB, IHC-P, IHC-F in Human;pig;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-NF68 Antibody (Monoclonal, NR4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1070)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Pig spinal cord.|
|Reactivity||Human, Pig, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, pig, rat, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, pig, rat, -
Western blot, 1-2μg/ml, Human, pig, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||Neurofilament light polypeptide|
|Alternative Names||Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl;|
|Molecular Weight||61335 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.|
|Research Areas||Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Developmental Biology, Intermediate Filaments, Lineage Markers, Lineage Specification, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells
*You can search these to find other products in these research areas.
|Background||Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM) and 200 kD(NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68(NF68), also called Neurofilament Protein, Light Chain(NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E(CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.|
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Order Product (MA1070)
Promotion:Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.