radio
SKU MA1070
Size 100μg/vial
Reactivity Human, Pig, Rat
Clonality Monoclonal
Clone Number NR4
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-NF68 Antibody (Monoclonal, NR4)
SKU/Catalog Number MA1070
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for NF68, neurofilament, light polypeptide (NEFL ) detection. Tested with WB, IHC-P, IHC-F in Human;pig;rat. No cross reactivity with other proteins.
Cite This Product Anti-NF68 Antibody (Monoclonal, NR4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1070)
Host Mouse
Immunogen Pig spinal cord.
Reactivity Human, Pig, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, pig, rat, By Heat
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, pig, rat, -
Western blot, 1-2μg/ml, Human, pig, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P19527
Gene Name Nefl
Protein Name Neurofilament light polypeptide
Alternative Names Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl;
Molecular Weight 61335 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Background Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM) and 200 kD(NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68(NF68), also called Neurofilament Protein, Light Chain(NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E(CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Order Product (MA1070)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.