|Product Name||Anti-Neuropilin 1 Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-Neuropilin 1 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IF, IHC, ICC, WB in Human, Mouse, Rat|
|Cite This Product||Anti-Neuropilin 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01324)|
|Immunogen||A synthesized peptide derived from human Neuropilin 1|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Flow Cytometry 1:50
Images And Assay Conditions
Western blot analysis of Neuropilin 1 expression in mouse heart lysate (M01324).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRP1 monoclonal antibody (Catalog # M01324) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRP1
Immunohistochemical analysis of paraffin-embedded human melanoma, using Neuropilin 1 Antibody (M01324)
NRP1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NRP1 Antibody (M01324)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.|
|Alternative Names||Neuropilin-1;Vascular endothelial cell growth factor 165 receptor;CD304;NRP1;NRP, VEGF165R;|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Molecular Weight||103134 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.|
|Research Areas||Adaptive Immunity, Angiogenesis, Axonal Guidance Proteins, Cardiovascular, Growth And Development, Growth Factors, Immunology, Neurology Process, Neuroscience
*You can search these to find other products in these research areas.
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