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- Table of Contents
Nucb2 antibody (PB9876) performs well in ELISA, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876).
Nucleobindin 2 was detected in a paraffin-embedded section of mouse gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleobindin 2 Antibody (PB9876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Nucleobindin 2 was detected in a paraffin-embedded section of mouse gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleobindin 2 Antibody (PB9876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-Nucleobindin 2/NUCB2 Antibody Picoband®
Nucb2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
Nucb2 antibody was positively detected by WB in:
HEPA whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Nucb2 antibody was positively detected by IHC in:
mouse gaster tissue, rat gaster tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
ELISA
0.1-0.5μg/ml
-
Western blot
0.1-0.5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Mouse
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Nucleobindin 2/NUCB2 Antibody Picoband®
View all NUCB2 Antibodies
View all NUCB2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
50.3 kDa
Background of Nucb2
Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and sequence analysis, the NUCB2 sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.
Cite This Product
Anti-Nucleobindin 2/NUCB2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9876)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
98%
High Homology
Human
85%
Moderate Homology
Rabbit
85%
Moderate Homology
Monkey
85%
Moderate Homology
Cow
84%
Moderate Homology
Dog
84%
Moderate Homology
Sheep
83%
Moderate Homology
Zebrafish
65%
Low homology
Drosophila
48%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Nucb2 antibody
Immunohistochemistry (IHC) protocol for Nucb2 antibody
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Product Promise
We promise Anti-Nucleobindin 2/NUCB2 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9876
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026