Anti-NF-1C NFIC Monoclonal Antibody

NFIC antibody

Boster Bio Anti-NF-1C NFIC Monoclonal Antibody catalog # A04154. Tested in WB applications. This antibody reacts with Bovine, Chicken, Human, Mouse, Pig, Rat.

Product Info Summary

SKU: A04154
Size: 100μl
Reactive Species: Bovine, Chicken, Human, Mouse, Pig, Rat
Host: Mouse
Application: WB

Customers Who Bought This Also Bought

Product Name

Anti-NF-1C NFIC Monoclonal Antibody

View all NFIC Antibodies

SKU/Catalog Number

A04154

Size

100μl

Form

Liquid

Description

Boster Bio Anti-NF-1C NFIC Monoclonal Antibody catalog # A04154. Tested in WB applications. This antibody reacts with Bovine, Chicken, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NF-1C NFIC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04154)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

5G7

Isotype

IgG

Immunogen

Purified recombinant human NF-1C protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04154 is reactive to NFIC in Bovine, Chicken, Human, Mouse, Pig, Rat

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

55675 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A04154 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For NFIC (Source: Uniprot.org, NCBI)

Gene Name

NFIC

Full Name

Nuclear factor 1 C-type

Weight

55675 MW

Superfamily

CTF/NF-I family

Alternative Names

Nuclear factor 1 C-type;NF1-C;Nuclear factor 1/C;CCAAT-box-binding transcription factor;CTF;Nuclear factor I/C;NF-I/C;NFI-C;TGGCA-binding protein;NFIC;NFI; NFIC CTF, CTF5, NF-I, NFI nuclear factor I C nuclear factor 1 C-type|CCAAT-box-binding transcription factor|NF-I/C|NF1-C|TGGCA-binding protein|nuclear factor I/C (CCAAT-binding transcription factor)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFIC, check out the NFIC Infographic

NFIC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFIC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A04154

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-NF-1C NFIC Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-NF-1C NFIC Monoclonal Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

13 Customer Q&As for Anti-NF-1C NFIC Monoclonal Antibody

Question

I was wanting to use your anti-NF-1C Monoclonal antibody for WB for chicken cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for chicken cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

As indicated on the product datasheet, A04154 anti-NF-1C Monoclonal antibody has been validated for WB on bovine, chicken, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in chicken cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-08

Question

My question regards to test anti-NF-1C Monoclonal antibody A04154 on chicken cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-NF-1C Monoclonal antibody A04154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Johnson

Verified customer

Asked: 2020-04-24

Answer

The products we sell, including anti-NF-1C Monoclonal antibody A04154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-24

Question

I see that the anti-NF-1C Monoclonal antibody A04154 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-21

Question

We are currently using anti-NF-1C Monoclonal antibody A04154 for pig tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, chicken, human, mouse, pig, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

The anti-NF-1C Monoclonal antibody (A04154) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-07

Question

Would anti-NF-1C Monoclonal antibody A04154 work on canine WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

Our lab technicians have not validated anti-NF-1C Monoclonal antibody A04154 on canine. You can run a BLAST between canine and the immunogen sequence of anti-NF-1C Monoclonal antibody A04154 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukemic t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-03

Question

Is this A04154 anti-NF-1C Monoclonal antibody reactive to the isotypes of NFIC?

S. Carter

Verified customer

Asked: 2018-09-26

Answer

The immunogen of A04154 anti-NF-1C Monoclonal antibody is Purified recombinant human NF-1C protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-09-26

Question

Do you have a BSA free version of anti-NF-1C Monoclonal antibody A04154 available?

T. Mangal

Verified customer

Asked: 2018-09-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NF-1C Monoclonal antibody A04154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-09-03

Question

Can you help my question with product A04154, anti-NF-1C Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Wu

Verified customer

Asked: 2017-11-02

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04154 anti-NF-1C Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-11-02

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NF-1C Monoclonal antibody A04154. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-28

Question

Is a blocking peptide available for product anti-NF-1C Monoclonal antibody (A04154)?

Verified Customer

Verified customer

Asked: 2017-07-12

Answer

We do provide the blocking peptide for product anti-NF-1C Monoclonal antibody (A04154). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-07-12

Question

See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NF-1C Monoclonal antibody A04154. Please let me know if you require anything else.

A. Jones

Verified customer

Asked: 2015-09-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-09-11

Question

Does anti-NF-1C Monoclonal antibody A04154 work for WB with cervix carcinoma erythroleukemia?

A. Wu

Verified customer

Asked: 2014-07-11

Answer

According to the expression profile of cervix carcinoma erythroleukemia, NFIC is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-NF-1C Monoclonal antibody A04154 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2014-07-11

Question

Would A04154 anti-NF-1C Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Anderson

Verified customer

Asked: 2014-01-24

Answer

As indicated on the product datasheet, A04154 anti-NF-1C Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-01-24

Order DetailsPrice
A04154

100uL

$399

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A04154
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.