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Product Info Summary
| SKU: | A04154 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Bovine, Chicken, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-NF-1C NFIC Monoclonal Antibody
SKU/Catalog Number
A04154
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NF-1C NFIC Monoclonal Antibody catalog # A04154. Tested in WB applications. This antibody reacts with Bovine, Chicken, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-1C NFIC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04154)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Purified recombinant human NF-1C protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04154 is reactive to NFIC in Bovine, Chicken, Human, Mouse, Pig, Rat
Applications
A04154 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
55.675kDa
Background of NFIC
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102(35): 12407-12412
Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280(3): 605-609
Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359(2): 151-159
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis using NF-1C Monoclonal antibody against HeLa nuclear extract.
Protein Target Info & Infographic
Gene/Protein Information For NFIC (Source: Uniprot.org, NCBI)
Gene Name
NFIC
Full Name
Nuclear factor 1 C-type
Weight
55.675kDa
Superfamily
CTF/NF-I family
Alternative Names
CCAAT-binding transcription factor; CTF5; CTFCCAAT-box-binding transcription factor; MGC20153; NF1-C; NFI; NF-I; NF-I/C; NFI-C; nuclear factor 1 C-type; Nuclear factor 1/C; nuclear factor I/C (CCAAT-binding transcription factor); Nuclear factor I/C; TGGCA-binding protein NFIC CTF, CTF5, NF-I, NFI nuclear factor I C nuclear factor 1 C-type|CCAAT-box-binding transcription factor|NF-I/C|NF1-C|TGGCA-binding protein|nuclear factor I/C (CCAAT-binding transcription factor)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFIC, check out the NFIC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFIC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-1C NFIC Monoclonal Antibody (A04154)
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No publications found for A04154
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
13 Customer Q&As for Anti-NF-1C NFIC Monoclonal Antibody
Question
I was wanting to use your anti-NF-1C Monoclonal antibody for WB for chicken cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for chicken cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
As indicated on the product datasheet, A04154 anti-NF-1C Monoclonal antibody has been validated for WB on bovine, chicken, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in chicken cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-08
Question
My question regards to test anti-NF-1C Monoclonal antibody A04154 on chicken cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-NF-1C Monoclonal antibody A04154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Johnson
Verified customer
Asked: 2020-04-24
Answer
The products we sell, including anti-NF-1C Monoclonal antibody A04154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-24
Question
I see that the anti-NF-1C Monoclonal antibody A04154 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-NF-1C Monoclonal antibody A04154 for pig tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, chicken, human, mouse, pig, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
The anti-NF-1C Monoclonal antibody (A04154) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-07
Question
Would anti-NF-1C Monoclonal antibody A04154 work on canine WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
Our lab technicians have not validated anti-NF-1C Monoclonal antibody A04154 on canine. You can run a BLAST between canine and the immunogen sequence of anti-NF-1C Monoclonal antibody A04154 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-03
Question
Is this A04154 anti-NF-1C Monoclonal antibody reactive to the isotypes of NFIC?
S. Carter
Verified customer
Asked: 2018-09-26
Answer
The immunogen of A04154 anti-NF-1C Monoclonal antibody is Purified recombinant human NF-1C protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-26
Question
Do you have a BSA free version of anti-NF-1C Monoclonal antibody A04154 available?
T. Mangal
Verified customer
Asked: 2018-09-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NF-1C Monoclonal antibody A04154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-03
Question
Can you help my question with product A04154, anti-NF-1C Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Wu
Verified customer
Asked: 2017-11-02
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04154 anti-NF-1C Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-02
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NF-1C Monoclonal antibody A04154. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-28
Question
Is a blocking peptide available for product anti-NF-1C Monoclonal antibody (A04154)?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
We do provide the blocking peptide for product anti-NF-1C Monoclonal antibody (A04154). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-07-12
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NF-1C Monoclonal antibody A04154. Please let me know if you require anything else.
A. Jones
Verified customer
Asked: 2015-09-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-11
Question
Does anti-NF-1C Monoclonal antibody A04154 work for WB with cervix carcinoma erythroleukemia?
A. Wu
Verified customer
Asked: 2014-07-11
Answer
According to the expression profile of cervix carcinoma erythroleukemia, NFIC is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-NF-1C Monoclonal antibody A04154 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2014-07-11
Question
Would A04154 anti-NF-1C Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Anderson
Verified customer
Asked: 2014-01-24
Answer
As indicated on the product datasheet, A04154 anti-NF-1C Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-01-24
