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Product Info Summary
| SKU: | A02727-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband®
SKU/Catalog Number
A02727-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband® catalog # A02727-3. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02727-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NFAT4/NF-ATc3/NFATC3 recombinant protein (Position: R32-Q880).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02727-3 is reactive to NFATC3 in Human
Observed Molecular Weight
150 kDa
Calculated molecular weight
115.6 kDa
Background of NFATC3
Nuclear factor of activated T-cells, cytoplasmic 3 is a protein that in humans is encoded by the NFATC3 gene. The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02727-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of NFAT4/NF-ATc3/NFATC3 using anti-NFAT4/NF-ATc3/NFATC3 antibody (A02727-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4/NF-ATc3/NFATC3 antigen affinity purified polyclonal antibody (Catalog # A02727-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4/NF-ATc3/NFATC3 at approximately 150 kDa. The expected band size for NFAT4/NF-ATc3/NFATC3 is at 116 kDa.
Specific Publications For Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband® (A02727-3)
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