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- Table of Contents
NFATC3 antibody (A02727-3) performs well in ELISA, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of NFAT4/NF-ATc3/NFATC3 using anti-NFAT4/NF-ATc3/NFATC3 antibody (A02727-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4/NF-ATc3/NFATC3 antigen affinity purified polyclonal antibody (Catalog # A02727-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4/NF-ATc3/NFATC3 at approximately 150 kDa. The expected band size for NFAT4/NF-ATc3/NFATC3 is at 116 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT4/NF-ATc3/NFATC3 antigen affinity purified polyclonal antibody (Catalog # A02727-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFAT4/NF-ATc3/NFATC3 at approximately 150 kDa. The expected band size for NFAT4/NF-ATc3/NFATC3 is at 116 kDa.
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Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband®
NFATC3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, WB |
Tested Applications
NFATC3 antibody was positively detected by WB in:
human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Bovine
Predicted
Predicted
Dog
Predicted
Predicted
Sheep
Predicted
Predicted
Rabbit
Predicted
Predicted
Rat
Predicted
Predicted
Mouse
Predicted
Predicted
Product Information
Product Name
Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband®
View all NFATC3 Antibodies
View all NFATC3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NFAT4/NF-ATc3/NFATC3 recombinant protein (Position: R32-Q880).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
115.6 kDa
Background of NFATC3
Nuclear factor of activated T-cells, cytoplasmic 3 is a protein that in humans is encoded by the NFATC3 gene. The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes.
Cite This Product
Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02727-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Cow
94%
High Homology
Dog
94%
High Homology
Sheep
94%
High Homology
Rabbit
92%
High Homology
Rat
91%
High Homology
Mouse
90%
High Homology
Zebrafish
65%
Low homology
Drosophila
19%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NFATC3 antibody
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Product Promise
We promise Anti-NFAT4/NF-ATc3/NFATC3 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02727-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026