Anti-NFS1 Antibody Picoband™

Product Name

Anti-NFS1 Antibody Picoband™

View all NFS1 Antibodies

SKU/Catalog Number

A05061-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NFS1 Antibody Picoband™ catalog # A05061-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NFS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05061-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human NFS1 recombinant protein (Position: R107-L356). Human NFS1 shares 96.4% and 96% amino acid (aa) sequence identity with mouse and rat NFS1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05061-1 is reactive to NFS1 in Human, Mouse, Rat

Applications

A05061-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

50.196kDa

Background of NFS1

Cysteine desulfurase, mitochondrial is an enzyme that in humans is encoded by the NFS1 gene. Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NFS1 using anti-NFS1 antibody (A05061-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse B16-F10 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFS1 antigen affinity purified polyclonal antibody (Catalog # A05061-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFS1 at approximately 45 kDa. The expected band size for NFS1 is at 50 kDa.

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Figure 2. Flow Cytometry analysis of HEL cells using anti-NFS1 antibody (A05061-1).
Overlay histogram showing HEL cells stained with A05061-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFS1 Antibody (A05061-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NFS1 (Source: Uniprot.org, NCBI)

Alternative Names

cysteine desulfurase, mitochondrial; EC 2.8.1.7; NFS1 nitrogen fixation 1 homolog (S. cerevisiae); NifS; NIFShomolog); nitrogen-fixing bacteria S-like protein NFS1 HUSSY-08, IscS, NIFS NFS1 cysteine desulfurase cysteine desulfurase, mitochondrial|NFS1 nitrogen fixation 1 homolog|nitrogen fixation 1 (S. cerevisiae, homolog)|nitrogen-fixing bacteria S-like protein

For more info on NFS1, check out the NFS1 Infographic

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In this infographic, you will see the following information for NFS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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