Product Info Summary
SKU: | A10259-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NGDN Antibody Picoband™
SKU/Catalog Number
A10259-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NGDN Antibody Picoband™ catalog # A10259-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NGDN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10259-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NGDN recombinant protein (Position: E8-K191).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10259-1 is reactive to NGDN in Human
Applications
A10259-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
35.894kDa
Background of NGDN
Neuroguidin is an EIF4E-binding protein that interacts with CPEB and functions as a translational regulatory protein during development of the vertebrate nervous system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NGDN using anti-NGDN antibody (A10259-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGDN antigen affinity purified polyclonal antibody (Catalog # A10259-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGDN at approximately 36 kDa. The expected band size for NGDN is at 36 kDa.
Click image to see more details
Figure 2. IF analysis of NGDN using anti-NGDN antibody (A10259-1).
NGDN was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NGDN Antibody (A10259-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of U251 cells using anti-NGDN antibody (A10259-1).
Overlay histogram showing U251 cells stained with A10259-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NGDN Antibody (A10259-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NGDN (Source: Uniprot.org, NCBI)
Gene Name
NGDN
Full Name
Neuroguidin
Weight
35.894kDa
Superfamily
SAS10 family
Alternative Names
C14orf120; DKFZP564O092; EIF4E-binding protein; eukaryotic initiation factor 4E and CPEB binding protein; LCP5; lpd-2; neuroguidin; neuroguidin, EIF4E binding protein; NGDchromosome 14 open reading frame 120 NGDN C14orf120, CANu1, LCP5, NGD, lpd-2 neuroguidin neuroguidin|EIF4E-binding protein|centromere accumulated nuclear protein 1|eukaryotic initiation factor 4E and CPEB binding protein|neuroguidin, EIF4E binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGDN, check out the NGDN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGDN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NGDN Antibody Picoband™ (A10259-1)
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