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Product Info Summary
| SKU: | M02427-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IP, IHC-P, WB |
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Product info
Product Name
Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody
SKU/Catalog Number
M02427-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody (Catalog# M02427-1). Tested in IP, WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02427-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EBP-10
Isotype
Mouse IgG1 kappa
Immunogen
Bacterially produced recombinant full-length human NHERF1. The mouse monoclonal antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02427-1 is reactive to SLC9A3R1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
38.9 kDa
Background of SLC9A3R1
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02427-1 is guaranteed for IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 5 μg/ml.
Western blotting: 2 μg/ml, reducing conditions, positive control: MCF-7 cell line
Validation Images & Assay Conditions
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Western blotting analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-NHERF1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50 kDa.
Specific Publications For Anti-NHERF1 Purified SLC9A3R1 Monoclonal Antibody (M02427-1)
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