Anti-Niemann Pick C2/NPC2 Antibody Picoband™

Niemann-Pick type C2 antibody

Boster Bio Anti-Niemann Pick C2/NPC2 Antibody Picoband™ catalog # A01582-2. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: A01582-2
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Niemann Pick C2/NPC2 Antibody Picoband™

View all Niemann-Pick type C2 Antibodies

SKU/Catalog Number

A01582-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Niemann Pick C2/NPC2 Antibody Picoband™ catalog # A01582-2. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Niemann Pick C2/NPC2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01582-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Niemann Pick C2, which shares 79.4% and 76.5% amino acid (aa) sequence identity with mouse and rat Niemann Pick C2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01582-2 is reactive to NPC2 in Human

Applications

A01582-2 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

16.57kDa

Background of Niemann-Pick type C2

NPC2 is a protein associated with Niemann-Pick disease, type C. This gene is mapped to chromosome 14q24.3. It encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section),0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For NPC2 (Source: Uniprot.org, NCBI)

Gene Name

NPC2

Full Name

NPC intracellular cholesterol transporter 2

Weight

16.57kDa

Superfamily

NPC2 family

Alternative Names

EDDM1; Epididymal Protein 1; epididymal secretory protein E1; HE1; HE1epididymal protein 1; Human epididymis-specific protein 1; MGC1333; Niemann-Pick disease type C2 protein; Niemann-Pick disease, type C2; NiemannPick Type C2; Niemann-Pick Type C2; NPC2; NP-C2; tissue-specific secretory protein NPC2 EDDM1, HE1 NPC intracellular cholesterol transporter 2 NPC intracellular cholesterol transporter 2|Niemann-Pick disease type C2 protein|epididymal protein 1|epididymis secretory sperm binding protein|human epididymis-specific protein 1|tissue-specific secretory protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NPC2, check out the NPC2 Infographic

NPC2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NPC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01582-2 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Identification of pimavanserin tartrate as a potent Ca2+-calcineurin-NFAT pathway inhibitor for glioblastoma therapy

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6 Customer Q&As for Anti-Niemann Pick C2/NPC2 Antibody Picoband™

Question

Do you have a BSA free version of anti-Niemann Pick C2/NPC2 antibody A01582-2 available?

J. Wu

Verified customer

Asked: 2019-10-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Niemann Pick C2/NPC2 antibody A01582-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-17

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-Niemann Pick C2/NPC2 antibody A01582-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-23

Question

We are currently using anti-Niemann Pick C2/NPC2 antibody A01582-2 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

The anti-Niemann Pick C2/NPC2 antibody (A01582-2) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-26

Question

I was wanting to use your anti-Niemann Pick C2/NPC2 antibody for WB for human ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovary identification?

Verified Customer

Verified customer

Asked: 2019-02-20

Answer

As indicated on the product datasheet, A01582-2 anti-Niemann Pick C2/NPC2 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-20

Question

Is a blocking peptide available for product anti-Niemann Pick C2/NPC2 antibody (A01582-2)?

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

We do provide the blocking peptide for product anti-Niemann Pick C2/NPC2 antibody (A01582-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-29

Question

My question regarding product A01582-2, anti-Niemann Pick C2/NPC2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01582-2 anti-Niemann Pick C2/NPC2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-01-25

Order DetailsPrice
A01582-2

100μg

$370
A01582-2-10ug

10μg sample (liquid)

$99
A01582-2-Biotin

100 μg Biotin conjugated

$570
A01582-2-Cy3

100 μg Cy3 conjugated

$570
A01582-2-Dylight488

100 μg Dylight488 conjugated

$570
A01582-2-Dylight550

100 μg Dylight550 conjugated

$570
A01582-2-Dylight594

100 μg Dylight594 conjugated

$570
A01582-2-FITC

100 μg FITC conjugated

$570
A01582-2-HRP

100 μg HRP conjugated

$570
A01582-2-APC

100 μg APC conjugated

$670
A01582-2-PE

100 μg PE conjugated

$670
A01582-2-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A01582-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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