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Product Info Summary
| SKU: | A08377 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P |
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Product info
Product Name
Anti-Ninjurin-1 NINJ1 Antibody
SKU/Catalog Number
A08377
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ninjurin-1 NINJ1 Antibody (Catalog # A08377). Tested in ELISA, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NINJ1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Ninjurin-1 NINJ1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08377)
Host
Rabbit
Contents
NINJ1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NINJ1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NINJ1. The immunogen is located within the last 50 amino acids of NINJ1.
Cross-reactivity
NINJ1 antibody is human, mouse and rat reactive.
Reactive Species
A08377 is reactive to NINJ1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
16.3 kDa
Background of NINJ1
Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08377 is guaranteed for ELISA, IF, IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NINJ1 antibody can be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunohistochemistry of NINJ1 in mouse brain tissue with NINJ1 antibody at 5 μg/ml.
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Immunofluorescence of NINJ1 in mouse brain tissue with NINJ1 antibody at 20 μg/ml.
Specific Publications For Anti-Ninjurin-1 NINJ1 Antibody (A08377)
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