Product Info Summary
SKU: | A07135S350 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-NIPA (S350) ZC3HC1 Antibody
SKU/Catalog Number
A07135S350
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NIPA (S350) ZC3HC1 Antibody catalog # A07135S350. Tested in WB,IF applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NIPA (S350) ZC3HC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07135S350)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TAF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07135S350 is reactive to ZC3HC1 in Human, Mouse
Applications
A07135S350 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.262kDa
Background of NIPA
Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.
Gangloff Y.G., Mol. Cell. Biol. 21:5109-5121(2001) [PubMed: 11438666].
Deloukas P., Nature 429:375-381(2004) [PubMed: 15164054].
Bechtel S., BMC Genomics 8:399-399(2007) [PubMed: 17974005].
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-NIPA (S350) ZC3HC1 Antibody A07135S350
Western blot (WB) analysis of NIPA (S350) pAb at 1:500 dilutionLane1:PC3 whole cell lysate(20ug)
Lane2:K562 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(10ug)
Lane4:AML-12 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 2. Western blotting validation for Anti-NIPA (S350) ZC3HC1 Antibody A07135S350
Western blot (WB) analysis of NIPA (S350) pAb at 1:500 dillutionLane1:HEK293T whole cell lysate
Lane2:U-87MG whole cell lysate
Lane3:H9C2 whole cell lysate
Lane4:CT-26 whole cell lysate
Lane5:The Testis tissue lysate of Mouse
Lane6:The Testis tissue lysate of Rat
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ZC3HC1 (Source: Uniprot.org, NCBI)
Gene Name
ZC3HC1
Full Name
Nuclear-interacting partner of ALK
Weight
55.262kDa
Alternative Names
C3HC type 1; FBXO50; zinc finger, C3HC-type containing 1 ZC3HC1 HSPC216, NIPA zinc finger C3HC-type containing 1 nuclear-interacting partner of ALK|hematopoietic stem/progenitor cell protein 216|nuclear interacting partner of anaplastic lymphoma kinase (ALK)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZC3HC1, check out the ZC3HC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZC3HC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NIPA (S350) ZC3HC1 Antibody (A07135S350)
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No publications found for A07135S350
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