This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SLC12A2 antibody (M03603) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (M03603).
NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-NKCC1/SLC12A2 Antibody (M03603) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2)
SLC12A2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IF, IHC, WB |
Tested Applications
SLC12A2 antibody was positively detected by WB in:
human A549 whole cell, human CACO-2 whole cell, human PC-3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC12A2 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC12A2 antibody was positively detected by IF in:
human colorectal adenocarcinoma tissue, mouse colon tissue, rat colon tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunofluorescence
5 μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Rat
Tested
Tested
Rabbit
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2)
View all SLC12A2 Antibodies
View all SLC12A2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6G7D2
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human NKCC1/SLC12A2 recombinant protein (Position: K889-K943).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
200 kDa
Calculated Molecular Weight
131.4 kDa
Background of SLC12A2
Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
Cite This Product
Anti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03603)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Monkey
100%
High Homology
Mouse
98%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Dog
98%
High Homology
Zebrafish
56%
Low homology
Drosophila
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC12A2 antibody
Immunohistochemistry (IHC) protocol for SLC12A2 antibody
Immunofluorescence (IF) protocol for SLC12A2 antibody
Publications
Application:
Species:
High ambient temperature may induce presbyopia via TRPV1 activation
Published: 2024-07-09
Journal: Medical Molecular Morphology
Application: WB,IF
Impact Factor: 1.20
Species: Human
PMID: 38980406
Review
Have you used Anti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-NKCC1/SLC12A2 Antibody Picoband® (monoclonal, 6G7D2) performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M03603
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026