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Product Info Summary
| SKU: | A16315 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-NKX1-1 Antibody (Center)
SKU/Catalog Number
A16315
Size
80 µl
Description
Boster Bio Anti-NKX1-1 Antibody (Center) (Catalog # A16315). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-NKX1-1 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16315)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This NKX1-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the Central region of human NKX1-1.
Reactive Species
A16315 is reactive to NKX1-1 in Human
Calculated molecular weight
40.7 kDa
Background of NKX1-1
The function of this protein remains unknown.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16315 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
Validation Images & Assay Conditions
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Fluorescent confocal image of Hela cell stained with NKX1-1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.
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NKX1-1 Antibody (Center) (Cat. #A16315) western blot analysis in SK-N-MC cell line lysates (35ug/lane). This demonstrates the NKX1-1 antibody detected the NKX1-1 protein (arrow).
Specific Publications For Anti-NKX1-1 Antibody (Center) (A16315)
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