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Product Info Summary
| SKU: | A00525-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-NLRC4 Antibody
SKU/Catalog Number
A00525-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NLRC4 Antibody catalog # A00525-1. Tested in ELISA,IHC,ICC,IF applications. This antibody reacts with Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NLRC4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00525-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
KLH conjugated synthetic peptide derived from human CARD12:401-500/1024.
Reactive Species
A00525-1 is reactive to NLRC4 in Rat
Calculated molecular weight
116.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00525-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5000-10000
IHC: 1:100-500
ICC: 1:100-500
IF: 1:100-500
Validation Images & Assay Conditions
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NLRC4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Specific Publications For Anti-NLRC4 Antibody (A00525-1)
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