Anti-NLRP9 Antibody Picoband™

Product Name

Anti-NLRP9 Antibody Picoband™

View all NLRP9 Antibodies

SKU/Catalog Number

A14119-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NLRP9 Antibody Picoband™ catalog # A14119-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NLRP9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14119-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NLRP9 recombinant protein (Position: H118-H746).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A14119-1 is reactive to NLRP9 in Human

Applications

A14119-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

113 kDa

Calculated molecular weight

113.312kDa

Background of NLRP9

The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NLRP9 using anti-NLRP9 antibody (A14119-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP9 antigen affinity purified polyclonal antibody (Catalog # A14119-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP9 at approximately 113 kDa. The expected band size for NLRP9 is at 113 kDa.

Gene/Protein Information For NLRP9 (Source: Uniprot.org, NCBI)

Alternative Names

NACHT, LRR and PYD domains-containing protein 9 NLRP9 CLR19.1, NALP9, NOD6, PAN12 NLR family pyrin domain containing 9 NACHT, LRR and PYD domains-containing protein 9|NACHT, LRR and PYD containing protein 9|NACHT, leucine rich repeat and PYD containing 9|PYRIN and NACHT-containing protein 12|nucleotide-binding oligomerization domain protein 6|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 9

For more info on NLRP9, check out the NLRP9 Infographic

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In this infographic, you will see the following information for NLRP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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