Product Info Summary
SKU: | P01949 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-NMDA Receptor NR2A Subunit (Tyr1325) GRIN2A Antibody
SKU/Catalog Number
P01949
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-NMDA Receptor NR2A Subunit (Tyr1325) GRIN2A Antibody (Catalog # P01949). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-NMDA Receptor NR2A Subunit (Tyr1325) GRIN2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01949)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01949 is reactive to GRIN2A in Human, Mouse, Rat
Applications
P01949 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
165.283kDa
Background of GRIN2A
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr-1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).
Protein Target Info & Infographic
Gene/Protein Information For GRIN2A (Source: Uniprot.org, NCBI)
Gene Name
GRIN2A
Full Name
Glutamate receptor ionotropic, NMDA 2A
Weight
165.283kDa
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2A; hNR2A; NMDA receptor subtype 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; subunit epsilon-1 GRIN2A EPND, FESD, GluN2A, LKS, NMDAR2A, NR2A glutamate ionotropic receptor NMDA type subunit 2A glutamate receptor ionotropic, NMDA 2A|N-methyl D-aspartate receptor subtype 2A|N-methyl-D-aspartate receptor channel, subunit epsilon-1|N-methyl-D-aspartate receptor subunit 2A|glutamate ionotropic receptor NMDA 2A|glutamate receptor, ionotropic, N-methyl D-aspartate 2A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN2A, check out the GRIN2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-NMDA Receptor NR2A Subunit (Tyr1325) GRIN2A Antibody (P01949)
Hello CJ!
P01949 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liang Y,Ma Y,Wang J,Nie L,Hou X,Wu W,Zhang X,Tian Y. Leptin Contributes to Neuropathic Pain via Extrasynaptic NMDAR-nNOS Activation. Mol Neurobiol.2020 Oct 25.doi:10.1007/s12035-020-02180-1.Epub ahead of print.PMID:33099751.
Species: Rat
P01949 usage in article: APP:WB, SAMPLE:DRG NEURONS, DILUTION:1:400
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