Click image to see more details
Product Info Summary
| SKU: | A01808-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF |
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Product info
Product Name
Anti-NMDAR1 (Ab-897) GRIN1 Antibody
SKU/Catalog Number
A01808-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-NMDAR1 (Ab-897) GRIN1 Antibody (Catalog # A01808-1). Tested in IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NMDAR1 (Ab-897) GRIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01808-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.895~899 (R-S-S-K-D) derived from Human NMDAR1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01808-1 is reactive to GRIN1 in Human, Mouse, Rat
Applications
A01808-1 is guaranteed for IF Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
105.373kDa
Background of GRIN1
NMDA receptors are members of the ionotropic class of glutamate receptors, which also includes Kainate and AMPA receptors. NMDA receptors consist of NR1 subunits combined with one or more NR2 (A-D) or NR3 (A-B) subunits. The ligand-gated channel is permeable to cations including Ca2+, and at resting membrane potentials NMDA receptors are inactive due to a voltage-dependent blockade of the channel pore by Mg2+. NMDA receptor activation, which requires binding of glutamate and glycine, leads to an influx of Ca2+ into the postsynaptic region where it activates several signaling cascades, including pathways leading to the induction of long-term potentiation (LTP) and depression (LTD). NMDA receptors have a critical role in excitatory synaptic transmission and plasticity in the CNS. They govern a range of physiological conditions including neurological disorders caused by excitotoxic neuronal injury, psychiatric disorders and neuropathic pain syndromes.
Tyszkiewicz JP, et al. J Physiol. 2004 Feb 1; 554(Pt 3): 765-777
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 120kd
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using NMDAR1(Ab-897) Antibody #A01808-1.
Protein Target Info & Infographic
Gene/Protein Information For GRIN1 (Source: Uniprot.org, NCBI)
Gene Name
GRIN1
Full Name
Glutamate receptor ionotropic, NMDA 1
Weight
105.373kDa
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
glutamate [NMDA] receptor subunit zeta-1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; NMDA1; NMDAR1glutamate [NMDA] receptor subunit zeta 1; NMD-R1; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1 GRIN1 GluN1, MRD8, NDHMSD, NDHMSR, NMD-R1, NMDA1, NMDAR1, NR1 glutamate ionotropic receptor NMDA type subunit 1 glutamate receptor ionotropic, NMDA 1|N-methyl-D-aspartate receptor channel, subunit zeta-1|N-methyl-D-aspartate receptor subunit NR1|glutamate [NMDA] receptor subunit zeta-1|glutamate receptor, ionotropic, N-methyl D-aspartate 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIN1, check out the GRIN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NMDAR1 (Ab-897) GRIN1 Antibody (A01808-1)
Hello CJ!
A01808-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-NMDAR1 (Ab-897) GRIN1 Antibody
Question
Does A01808-1 anti-NMDAR1 (Ab-897) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
It shows on the product datasheet, A01808-1 anti-NMDAR1 (Ab-897) antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-06
Question
Does anti-NMDAR1 (Ab-897) antibody A01808-1 work for IF with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
According to the expression profile of anterior cingulate cortex, GRIN1 is highly expressed in anterior cingulate cortex. So, it is likely that anti-NMDAR1 (Ab-897) antibody A01808-1 will work for IF with anterior cingulate cortex.
Boster Scientific Support
Answered: 2020-02-04
Question
We have seen staining in mouse anterior cingulate cortex. Are there any suggestions? Is anti-NMDAR1 (Ab-897) antibody supposed to stain anterior cingulate cortex positively?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
From what I have seen in literature anterior cingulate cortex does express GRIN1. From what I have seen in Uniprot.org, GRIN1 is expressed in anterior cingulate cortex, brain, cerebellum hippocampus, among other tissues. Regarding which tissues have GRIN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7685113, 8406025
Cerebellum, and Hippocampus, Pubmed ID: 7926821
Boster Scientific Support
Answered: 2019-12-24
Question
I was wanting to use your anti-NMDAR1 (Ab-897) antibody for IF for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse anterior cingulate cortex identification?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
As indicated on the product datasheet, A01808-1 anti-NMDAR1 (Ab-897) antibody has been validated for IF on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-18
Question
I see that the anti-NMDAR1 (Ab-897) antibody A01808-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-17
Question
We are currently using anti-NMDAR1 (Ab-897) antibody A01808-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
The anti-NMDAR1 (Ab-897) antibody (A01808-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-04
Question
My boss were happy with the WB result of your anti-NMDAR1 (Ab-897) antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is GRIN1 supposed to be expressed?
K. Roberts
Verified customer
Asked: 2018-11-16
Answer
From what I have seen in literature, brain does express GRIN1. Generally GRIN1 expresses in cell membrane. Regarding which tissues have GRIN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7685113, 8406025
Cerebellum, and Hippocampus, Pubmed ID: 7926821
Boster Scientific Support
Answered: 2018-11-16
Question
I am interested in using your anti-NMDAR1 (Ab-897) antibody for excitatory postsynaptic potential studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
A. Moore
Verified customer
Asked: 2018-05-04
Answer
Thank you for your inquiry. This A01808-1 anti-NMDAR1 (Ab-897) antibody is validated on hela cells. It is guaranteed to work for IF in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-04
Question
See below the WB image, lot number and protocol we used for anterior cingulate cortex using anti-NMDAR1 (Ab-897) antibody A01808-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-22
Question
Do you have a BSA free version of anti-NMDAR1 (Ab-897) antibody A01808-1 available?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NMDAR1 (Ab-897) antibody A01808-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-05
Question
I have a question about product A01808-1, anti-NMDAR1 (Ab-897) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01808-1 anti-NMDAR1 (Ab-897) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-NMDAR1 (Ab-897) antibody A01808-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-09
Question
Is a blocking peptide available for product anti-NMDAR1 (Ab-897) antibody (A01808-1)?
D. Williams
Verified customer
Asked: 2017-06-30
Answer
We do provide the blocking peptide for product anti-NMDAR1 (Ab-897) antibody (A01808-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-30
Question
I am interested in to test anti-NMDAR1 (Ab-897) antibody A01808-1 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-NMDAR1 (Ab-897) antibody A01808-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Carter
Verified customer
Asked: 2014-10-14
Answer
The products we sell, including anti-NMDAR1 (Ab-897) antibody A01808-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-10-14
Question
Is this A01808-1 anti-NMDAR1 (Ab-897) antibody reactive to the isotypes of GRIN1?
D. Baker
Verified customer
Asked: 2014-08-20
Answer
The immunogen of A01808-1 anti-NMDAR1 (Ab-897) antibody is Peptide sequence around aa.895~899 (R-S-S-K-D) derived from Human NMDAR1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-20
