|Validated Species:||Human, Mouse, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-NME2/Nm23 H2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase B(NME2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-NME2/Nm23 H2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1830)|
|Replacement Item||This antibody may replace the following items: sc-100400|sc-136141|sc-14789|sc-14790|sc-166937|sc-17587|sc-28829|sc-343|sc-514515|sc-514515-X|sc-67799 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human NME2(137-152aa EELVDYKSCAHDWVYE), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Nucleoside diphosphate kinase B(NDK B)|
|Molecular Weight||17298 MW|
|Protein Function||Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. .|
|Tissue Specificity||Ubiquitously expressed. .|
|Subcellular Localization||Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.|
|Alternative Names||Nucleoside diphosphate kinase B;NDK B;NDP kinase B;18.104.22.168;C-myc purine-binding transcription factor PUF;Histidine protein kinase NDKB;22.214.171.124;nm23-H2;NME2;NM23B;|
|Research Areas|||signal transduction|second messenger|nucleotide messenger|gtp| signal transduction|metabolism|energy metabolism| neuroscience|neurology process|neurogenesis| cancer|oncoproteins/suppressors|tumor suppressors| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|types of disease||
Background for Nucleoside diphosphate kinase B(NDK B)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NME2/Nm23 H2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NME2/Nm23 H2 Antibody Images
Click the images to enlarge.
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Liver Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: PANC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: U87 Cell Lysate
Lane 9: COLO320 Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Rat Cerebellum Tissue
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,