Anti-NogoA RTN4 Antibody

Product Name

Anti-NogoA RTN4 Antibody

View all RTN4 Antibodies

SKU/Catalog Number

A01844

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NogoA RTN4 Antibody (Catalog # A01844). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NogoA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NogoA RTN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01844)

Host

Rabbit

Contents

NogoA Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA. The immunogen is located within amino acids 200 - 250 of NogoA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA and NogoE.

Reactive Species

A01844 is reactive to RTN4 in Human, Mouse, Rat

Applications

A01844 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

129.931kDa

Background of RTN4

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of NogoA in human brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 μg/mL.

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Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 μg/mL.

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Immunofluorescence of NogoA in Mouse Brain tissue with NogoA antibody at 20 μg/mL.

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Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 5 μg/ml.

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Immunohistochemistry of NogoA in mouse brain tissue with NogoA Antibody at 5 μg/mL.

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Immunofluorescence of NogoA in mouse brain tissue with NogoA Antibody at 20 μg/mL.

Green: NogoA antibody (A01844)
Red: Phylloidin staining
Blue: DAPI staining

Gene/Protein Information For RTN4 (Source: Uniprot.org, NCBI)

Alternative Names

ASY; foocen; Human NogoA; KIAA0886; My043 protein; Nbla00271; Nbla10545; neurite growth inhibitor 220; Neurite outgrowth inhibitor; Neuroendocrine-specific protein C homolog; Neuroendocrine-specific protein; NI220/250; Nogo protein; NOGO-A; Nogo-B; NOGOC; Nogo-C; NOGORTN4-B1; NSP; NSP-CL; reticulon 4; reticulon 5; reticulon-4; reticulon-5; RTN4-A; RTN4-B2; RTN4-C; RTN-x RTN4 ASY, NI220/250, NOGO, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X-A, RTN4-B1, RTN4-B2, RTN4-C, RTN4 reticulon 4 reticulon-4|Human NogoA|My043 protein|foocen|neurite growth inhibitor 220|neurite outgrowth inhibitor|neuroendocrine-specific protein C homolog|reticulon 5

For more info on RTN4, check out the RTN4 Infographic

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In this infographic, you will see the following information for RTN4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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