Anti-NOX2 CYBB Antibody

Product Name

Anti-NOX2 CYBB Antibody

View all CYBB Antibodies

SKU/Catalog Number

A00328-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NOX2 CYBB Antibody (Catalog # A00328-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-NOX2 CYBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00328-1)

Host

Rabbit

Contents

NOX2 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2.

Cross-reactivity

NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.

Reactive Species

A00328-1 is reactive to CYBB in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

65.3 kDa

Background of CYBB

The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer's and Parkinson's disease (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00328-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CYBB

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