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- Table of Contents
CYBB antibody (A00328-1) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.
Designing a Western blot for CYBB? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CYBB Western Blot GuideBundle Save
Anti-NOX2 CYBB Antibody
CYBB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-NOX2 CYBB Antibody
View all CYBB Antibodies
View all CYBB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
NOX2 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
NOX2 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2.
Cross-Reactivity
NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
65.3 kDa
Background of CYBB
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer's and Parkinson's disease (3).
Cite This Product
Anti-NOX2 CYBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00328-1)
Publications
Application:
Species:
Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
Species: Rat
PMID: 32112504
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Product Promise
We promise Anti-NOX2 CYBB Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00328-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026