Product Info Summary
SKU: | A00328-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-NOX2 CYBB Antibody
SKU/Catalog Number
A00328-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NOX2 CYBB Antibody (Catalog # A00328-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-NOX2 CYBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00328-1)
Host
Rabbit
Contents
NOX2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
SK7
Isotype
IgG
Immunogen
Rabbit polyclonal NOX2 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX2. The immunogen is located within amino acids 140 - 190 of NOX2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
Reactive Species
A00328-1 is reactive to CYBB in Human, Mouse, Rat
Applications
A00328-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
65.336kDa
Background of CYBB/NOX2
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer's and Parkinson's disease (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NOX2 in rat brain tissue lysate with NOX2 antibody at 1 μg/ml.
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Immunohistochemistry of NOX2 in rat brain tissue with NOX2 antibody at 5 μg/ml.
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Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.
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Immunohistochemistry of NOX2 in rat brain tissue cells with NOX2 antibody at 2 μg/ml.
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Immunofluorescence of NOX2 in rat brain tissue with NOX2 antibody at 20 μg/ml.
Green: NOX2 Antibody (A00328-1)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For CYBB (Source: Uniprot.org, NCBI)
Gene Name
CYBB
Full Name
Cytochrome b-245 heavy chain
Weight
65.336kDa
Alternative Names
CGD; CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; EC 1.6.3; GP91-1; GP91PHOX; GP91-PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; NOX2chronic granulomatous disease; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit CYBB AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX cytochrome b-245 beta chain cytochrome b-245 heavy chain|CGD91-phox|NADPH oxidase 2|cytochrome b(558) subunit beta|cytochrome b-245 beta polypeptide|cytochrome b558 subunit beta|heme-binding membrane glycoprotein gp91phox|neutrophil cytochrome b 91 kDa polypeptide|p22 phagocyte B-cytochrome|superoxide-generating NADPH oxidase heavy chain subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYBB, check out the CYBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOX2 CYBB Antibody (A00328-1)
Hello CJ!
A00328-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
Species: Rat
A00328-1 usage in article: APP:WB, SAMPLE:KIDNEY,HEART MUSCLE AND VASCULAR TISSUES, DILUTION:NA
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