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Product Info Summary
| SKU: | M03589 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-NPRC Rabbit Monoclonal Antibody
View all Natriuretic Peptide Receptor C Antibodies
SKU/Catalog Number
M03589
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NPRC Rabbit Monoclonal Antibody catalog # M03589. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NPRC Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03589)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEDH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Natriuretic Peptide Receptor C
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03589 is reactive to NPR3 in Human, Mouse, Rat
Applications
M03589 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
28 kDa
Calculated molecular weight
59.808kDa
Background of Natriuretic Peptide Receptor C
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:100
Validation Images & Assay Conditions
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Western blot analysis of Natriuretic Peptide Receptor C expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NPR3 (Source: Uniprot.org, NCBI)
Gene Name
NPR3
Full Name
Atrial natriuretic peptide receptor 3
Weight
59.808kDa
Superfamily
ANF receptor family
Alternative Names
ANP-C; ANPR-C; ANPRCFLJ14054; Atrial natriuretic peptide clearance receptor; atrial natriuretic peptide receptor 3; Atrial natriuretic peptide receptor type C; C5orf23; chromosome 5 open reading frame 23; GUCY2B; lgj; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C); NPR3; NPRC; NPR-C; stri NPR3 ANP-C, ANPR-C, ANPRC, C5orf23, GUCY2B, NPR-C, NPRC natriuretic peptide receptor 3 atrial natriuretic peptide receptor 3|atrial natriuretic peptide clearance receptor|atrial natriuretic peptide receptor type C|atrionatriuretic peptide receptor C|guanylate cyclase C|natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPR3, check out the NPR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NPRC Rabbit Monoclonal Antibody (M03589)
Hello CJ!
No publications found for M03589
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-NPRC Rabbit Monoclonal Antibody
Question
Is this M03589 anti-NPRC Monoclonal antibody reactive to the isotypes of NPR3?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The immunogen of M03589 anti-NPRC Monoclonal antibody is A synthesized peptide derived from human Natriuretic Peptide Receptor C Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-22
Question
What sample was used in your WB analysis of M03589?
Verified customer
Asked: 2020-01-09
Answer
For the Anti-NPRC Rabbit Monoclonal Antibody (M03589), Western blot analysis of Natriuretic Peptide Receptor C expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-NPRC Monoclonal antibody M03589 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The anti-NPRC Monoclonal antibody (M03589) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-02
Question
My question regarding product M03589, anti-NPRC Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Krishna
Verified customer
Asked: 2017-05-24
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03589 anti-NPRC Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-24
Question
I see that the anti-NPRC Monoclonal antibody M03589 works with WB, what is the protocol used to produce the result images on the product page?
C. Zhao
Verified customer
Asked: 2016-04-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-04-04
Question
I was wanting to use your anti-NPRC Monoclonal antibody for WB for mouse pericardium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse pericardium identification?
K. Parker
Verified customer
Asked: 2014-06-25
Answer
As indicated on the product datasheet, M03589 anti-NPRC Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse pericardium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-25
