Click image to see more details
Product Info Summary
| SKU: | M01332 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01332
BM5162 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody catalog # M01332. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01332)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABHO-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NR5A2
Reactive Species
M01332 is reactive to NR5A2 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
61.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01332 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: human MCF-7 whole cell, human A549 whole cell, human Caco-2 whole cell, human 293T whole cell, rat ovary tissue, rat liver tissue, mouse ovary tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of NR5A2 using anti-NR5A2 antibody (M01332).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse ovary tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR5A2 antigen affinity purified monoclonal antibody (M01332) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NR5A2 at approximately 70 kDa. The expected band size for NR5A2 is at 61 kDa.
Specific Publications For Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody (M01332)
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Customer Q&As
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5 Customer Q&As for Anti-NR5A2/Lrh 1 Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 available?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-30
Question
I have attached the WB image, lot number and protocol we used for liver using anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
I have a question about product M01332, anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01332 anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
You can see on the product datasheet, M01332 anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-01
Question
Does anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
Our lab technicians have not validated anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NR5A2/Lrh 1 Rabbit Monoclonal antibody M01332 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-05
