Click image to see more details
Product Info Summary
| SKU: | M03746 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-NRCAM Monoclonal Antibody
SKU/Catalog Number
M03746
BM5200 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NRCAM Monoclonal Antibody catalog # M03746. Tested in WB, IP applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NRCAM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03746)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACOH-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NRCAM Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
Reactive Species
M03746 is reactive to NRCAM in Human, Rat
Observed Molecular Weight
144 kDa
Calculated molecular weight
143.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03746 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of NRCAM using anti-NRCAM antibody (M03746).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRCAM antigen affinity purified monoclonal antibody (M03746) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NRCAM at approximately 144 kDa. The expected band size for NRCAM is at 144 kDa.
Specific Publications For Anti-NRCAM Monoclonal Antibody (M03746)
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3 Customer Q&As for Anti-NRCAM Monoclonal Antibody
Question
We are currently using anti-NRCAM Monoclonal antibody M03746 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The anti-NRCAM Monoclonal antibody (M03746) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-27
Question
I see that the anti-NRCAM Monoclonal antibody M03746 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-21
Question
Is this M03746 anti-NRCAM Monoclonal antibody reactive to the isotypes of NRCAM?
V. Roberts
Verified customer
Asked: 2013-01-30
Answer
The immunogen of M03746 anti-NRCAM Monoclonal antibody is A synthesized peptide derived from human NRCAM Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-30
