Product Info Summary
SKU: | A10304 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-NRG4 Antibody
SKU/Catalog Number
A10304
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NRG4 Antibody catalog # A10304. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NRG4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10304)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPL39.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10304 is reactive to Nrg4 in Human, Mouse, Rat
Applications
A10304 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
12.744kDa
Background of Nrg4
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Two transcript variants encoding distinct isoforms have been described. A pseudogene corresponding to this gene is found on chromosome 5q.
Choi D.K., Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Spirina O., Gene 261:229-234(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-NRG4 Antibody A10304
Western blot (WB) analysis of NRG4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For Nrg4 (Source: Uniprot.org, NCBI)
Gene Name
Nrg4
Full Name
Pro-neuregulin-4, membrane-bound isoform
Weight
12.744kDa
Superfamily
neuregulin family
Alternative Names
DKFZp779N0541; DKFZp779N1944; heregulin 4; HRG4pro-NRG4; neuregulin 4; pro-neuregulin-4, membrane-bound isoform; Pro-NRG4 Nrg4|AI552600|neuregulin 4|pro-neuregulin-4, membrane-bound isoform|pro-NRG4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nrg4, check out the Nrg4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nrg4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NRG4 Antibody (A10304)
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No publications found for A10304
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