Product Info Summary
SKU: | A32443-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NSD3 Antibody Picoband™
SKU/Catalog Number
A32443-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NSD3 Antibody Picoband™ catalog # A32443-1. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NSD3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32443-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NSD3 recombinant protein (Position: Q46-K1425).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A32443-1 is reactive to NSD3 in Human, Monkey
Applications
A32443-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
161.613kDa
Background of NSD3
Histone-lysine N-methyltransferase NSD3 is an enzyme that in humans is encoded by the WHSC1L1 gene. This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NSD3 using anti-NSD3 antibody (A32443-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSD3 antigen affinity purified polyclonal antibody (Catalog # A32443-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSD3 at approximately 90 kDa. The expected band size for NSD3 is at 90 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NSD3 (Source: Uniprot.org, NCBI)
Gene Name
NSD3
Full Name
Histone-lysine N-methyltransferase NSD3
Weight
161.613kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase NSD3 NSD3 KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328 nuclear receptor binding SET domain protein 3 histone-lysine N-methyltransferase NSD3|Wolf-Hirschhorn syndrome candidate 1-like 1|nuclear SET domain-containing protein 3|protein whistle
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NSD3, check out the NSD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NSD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NSD3 Antibody Picoband™ (A32443-1)
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No publications found for A32443-1
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