Anti-NSE/ENO2 Antibody

Enolase 2/Neuron-specific Enolase antibody

Boster Bio Anti-NSE/ENO2 Antibody catalog # PA1061. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1061
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-NSE/ENO2 Antibody

View all Enolase 2/Neuron-specific Enolase Antibodies

SKU/Catalog Number

PA1061

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NSE/ENO2 Antibody catalog # PA1061. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NSE/ENO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1061)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NSE (420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1061 is reactive to ENO2 in Human, Mouse, Rat

Applications

PA1061 is guaranteed for IHC, WB Boster Guarantee

Background of Enolase 2/Neuron-specific Enolase

NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ENO2 (Source: Uniprot.org, NCBI)

Uniprot ID

P09104

Gene ID

2026

Gene Name

ENO2

Full Name

Gamma-enolase

Weight

47.269kDa

Superfamily

enolase family

Alternative Names

2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific enolase; NSE ENO2 HEL-S-279, NSE enolase 2 gamma-enolase|2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|enolase 2 (gamma, neuronal)|epididymis secretory protein Li 279|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neuronal enriched enolase|neurone-specific enolase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ENO2, check out the ENO2 Infographic

ENO2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ENO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1061 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury

Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells

Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons

Protective effects of early hypoxic post-conditioning in cultured cortical neurons

HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways:

Stearic acid protects primary cultured cortical neurons against oxidative stress4

Protective effect of Angelica sinensis on cerebral neurons from rat embryos under hypoxia**

Differentiation of neural stem cells into Schwann-like cells in vitro

Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells

Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25%u201335)-induced neuronal injury

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1 Customer Q&As for Anti-NSE/ENO2 Antibody

Question

We are currently using anti-NSE/ENO2 antibody PA1061 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

The anti-NSE/ENO2 antibody (PA1061) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-18

Size

Conjugation

Total: $315

SKU:PA1061

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$50 fee for conjugation. Antibody size is reduced to 50ug.

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