Anti-NSE/ENO2 Antibody

Enolase 2/Neuron-specific Enolase antibody

Boster Bio Anti-NSE/ENO2 Antibody catalog # PA1061. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 40 publication(s).

Product Info Summary

SKU: PA1061
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-NSE/ENO2 Antibody

View all Enolase 2/Neuron-specific Enolase Antibodies

SKU/Catalog Number







Boster Bio Anti-NSE/ENO2 Antibody catalog # PA1061. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NSE/ENO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1061)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




A synthetic peptide corresponding to a sequence at the C-terminus of human NSE (420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1061 is reactive to ENO2 in Human, Mouse, Rat


PA1061 is guaranteed for IHC, WB Boster Guarantee

Background of Enolase 2/Neuron-specific Enolase

NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For ENO2 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name





enolase family

Alternative Names

2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific enolase; NSE ENO2 HEL-S-279, NSE enolase 2 gamma-enolase|2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|enolase 2 (gamma, neuronal)|epididymis secretory protein Li 279|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neuronal enriched enolase|neurone-specific enolase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ENO2, check out the ENO2 Infographic

ENO2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ENO2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1061 has been cited in 40 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells

Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25%u201335)-induced neuronal injury

%u03B2-Elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells

Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons

Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1? expression in the transition zone

HIF-1? effects on angiogenic potential in human small cell lung carcinoma

Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells

In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects

Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury

Injury of cortical neurons is caused by the advanced glycation end products-mediated pathway

Anti-Epileptic Effect of Ganoderma Lucidum Polysaccharides by Inhibition of Intracellular Calcium Accumulation and Stimulation of Expression of CaMKII ? in Epileptic Hippocampal Neurons

Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells

Zhou Sn, Li Cs, Liu Lq, Li Y, Wang Xf, Shen L. Histol Histopathol. 2011 May;26(5):571-9. Increased Expression Of Annexin A7 In Temporal Lobe Tissue Of Patients With Refractory Epilepsy.

He Y, Zeng Sy, Zhou Sw, Qian Gs, Peng K, Mo Zx, Zhou Jy. Fitoterapia. 2014 Oct;98:166-73. Doi: 10.1016/J.Fitote.2014.08.002. Epub 2014 Aug 7. Effects Of Rhynchophylline On Glun1 And Glun2B Expressions In Primary Cultured Hippocampal Neurons.

Huang H, Jiang L, Li S, Deng J, Li Y, Yao J, Li B, Zheng J. Biomicrofluidics. 2014 Feb 19;8(1):014108. Doi: 10.1063/1.4864235. Ecollection 2014. Using Microfluidic Chip To Form Brain-Derived Neurotrophic Factor Concentration Gradient For Studying ...

Wan J, Chai H, Yu Z, Ge W, Kang N, Xia W, Che Y. J Exp Clin Cancer Res. 2011 Aug 15;30:77. Doi: 10.1186/1756-9966-30-77. Hif-1?? Effects On Angiogenic Potential In Human Small Cell Lung Carcinoma.

Li K, Zheng S, Xiao X, Wang Q, Zhou Y, Chen L. J Pediatr Surg. 2007 Aug;42(8):1433-8. The Structural Characteristics And Expression Of Neuropeptides In The Esophagus Of Patients With Congenital Esophageal Atresia And Tracheoesophageal Fistula.

Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...

Du Y, Zou Y, Yu W, Shi R, Zhang M, Yang W, Duan J, Deng Y, Wang X, L?? Y. Brain Res. 2013 May 6;1509:79-85. Doi: 10.1016/J.Brainres.2013.03.005. Epub 2013 Mar 18. Expression Pattern Of Sorting Nexin 25 In Temporal Lobe Epilepsy: A Study On Patient...

Hu Wx, Xiang Q, Wen Z, He D, Wu Xm, Hu Gz. Mol Med Rep. 2014 Jun;9(6):2573-81. Doi: 10.3892/Mmr.2014.2105. Epub 2014 Apr 2. Neuroprotective Effect Of Atractylodes Macrocephalaon Polysaccharides In Vitro On Neuronal Apoptosis Induced By Hypoxia.

Wu J, Yang J, Liu Q, Wu S, Ma H, Cai Y. Biol Trace Elem Res. 2013 Apr;152(1):125-34. Doi: 10.1007/S12011-013-9601-3. Epub 2013 Jan 23. Lanthanum Induced Primary Neuronal Apoptosis Through Mitochondrial Dysfunction Modulated By Ca??

Zhang Z, Zhou X, Zhou X, Xu X, Liao M, Yan L, Lv R, Luo H. Neural Regen Res. 2012 May 5;7(13):971-7. Doi: 10.3969/J.Issn.1673-5374.2012.13.002. Methyl 3,4-Dihydroxybenzoate Promotes Neurite Outgrowth Of Cortical Neurons Cultured In Vitro.

Shao Jl, Wan Xh, Chen Y, Bi C, Chen Hm, Zhong Y, Heng Xh, Qian Jq. J Mol Neurosci. 2011 Mar;43(3):453-60. Doi: 10.1007/S12031-010-9464-4. Epub 2010 Oct 22. H2S Protects Hippocampal Neurons From Anoxia-Reoxygenation Through Camp-Mediated Pi3K/Akt/P...

Liu Q, Shen Y, Chen J, Gao X, Feng C, Wang L, Zhang Q, Jiang X. Pharm Res. 2012 Feb;29(2):546-58. Doi: 10.1007/S11095-011-0641-0. Epub 2011 Dec 14. Nose-To-Brain Transport Pathways Of Wheat Germ Agglutinin Conjugated Peg-Pla Nanoparticles.

Xiao F, Lin Lf, Cheng X, Gao Q, Luo Hm. Mol Med Rep. 2012 Mar;5(3):619-24. Doi: 10.3892/Mmr.2011.692. Epub 2011 Dec 1. Nogo-66 Receptor Activation Inhibits Neurite Outgrowth And Increases ??-Amyloid Protein Secretion Of Cortical Neurons.

Sun C, Shao J, Su L, Zhao J, Bi J, Yang S, Zhang S, Gao J, Miao J. Cell Transplant. 2013;22(6):961-75. Doi: 10.3727/096368912X657413. Epub 2012 Oct 1. Cholinergic Neuron-Like Cells Derived From Bone Marrow Stromal Cells Induced By Tricyclodecane-9...

Zhang X, Cai Z, Wang G, Wang H, Liu Z, Guo X, Yang C, Wang X, Wang H, Shu C, Xiao L. Behav Brain Res. 2013 Apr 15;243:165-70. Doi: 10.1016/J.Bbr.2013.01.001. Epub 2013 Jan 11. F-Actin May Play An Important Role In Il-1??-Stimulated Hippocampal Neu...

Liu X, Teng Z, Cui C, Wang R, Liu M, Zhang Y. J Mol Neurosci. 2014 Jan;52(1):124-30. Doi: 10.1007/S12031-013-0216-0. Epub 2014 Jan 10. Amyloid Beta-Derived Diffusible Ligands (Addls) Induce Abnormal Expression Of Insulin Receptors In Rat Hippocamp...

Cai Z, Zhang X, Wang G, Wang H, Liu Z, Guo X, Yang C, Wang X, Wang H, Shu C, Xiao L. Neuro Endocrinol Lett. 2014;35(1):13-9. Bdnf Attenuates Il-1??-Induced F-Actin Remodeling By Inhibiting Nf-??b Signaling In Hippocampal Neurons.

Pei H, Su X, Zhao L, Li H, Guo Y, Zhang M, Xin H. Int J Mol Sci. 2012;13(3):2551-62. Doi: 10.3390/Ijms13032551. Epub 2012 Feb 23. Primary Study For The Therapeutic Dose And Time Window Of Picroside Ii In Treating Cerebral Ischemic Injury In Rats.

Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.

Li F, Miao Zn, Xu Yy, Zheng Sy, Qin Md, Gu Yz, Zhang Xg. Mol Med Rep. 2012 Sep;6(3):625-30. Doi: 10.3892/Mmr.2012.968. Epub 2012 Jun 26. Transplantation Of Human Amniotic Mesenchymal Stem Cells In The Treatment Of Focal Cerebral Ischemia.

Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...

Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...

Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.

Meng Xt, Chen D, Dong Zy, Liu Jm. Cell Biol Int. 2007 Jul;31(7):691-8. Epub 2007 Jan 19. Enhanced Neural Differentiation Of Neural Stem Cells And Neurite Growth By Amniotic Epithelial Cell Co-Culture.

Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W. J Mol Med (Berl). 2010 Nov;88(11):1181-90. Doi: 10.1007/S00109-010-0659-5. Epub 2010 Aug 10. Isolation Of Cancer Stem Cells From Transformed Human Mesenchymal Stem Cell Line F6.

Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.

Xu Yl, Sun Jg, Wu Fb, Xi Ym. J Stem Cells Regen Med. 2011 Oct 30;7(2):69-74. Ecollection 2011. Investigation Of Characteristics Of Feather Follicle Stem Cells And Their Regeneration Potential.

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1 Customer Q&As for Anti-NSE/ENO2 Antibody


We are currently using anti-NSE/ENO2 antibody PA1061 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-18


The anti-NSE/ENO2 antibody (PA1061) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-18


Total: $315


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