SKU PA1061
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-NSE/ENO2 Antibody
SKU/Catalog Number PA1061
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-NSE/ENO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1061)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human NSE(420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Anti-NSE antibody, PA1061, Western blotting
WB: Rat Brain Tissue Lysate

Figure 2. IHC analysis of NSE using anti-NSE antibody (PA1061).
NSE was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (PA1061) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P09104
Gene Name ENO2
Protein Name Gamma-enolase
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Alternative Names Gamma-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE;ENO2;
Subcellular Localization Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .
Molecular Weight 47269 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background NSE(neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

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Polyclonal antibody for NEURON SPECIFIC ENOLASE/ENO2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NEURON SPECIFIC ENOLASE/ENO2 information: Molecular Weight: 47269 MW; Subcellular Localization: Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form; Tissue Specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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In stock
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PA1061
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells
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He Y, Zeng Sy, Zhou Sw, Qian Gs, Peng K, Mo Zx, Zhou Jy. Fitoterapia. 2014 Oct;98:166-73. Doi: 10.1016/J.Fitote.2014.08.002. Epub 2014 Aug 7. Effects Of Rhynchophylline On Glun1 And Glun2B Expressions In Primary Cultured Hippocampal Neurons.
Huang H, Jiang L, Li S, Deng J, Li Y, Yao J, Li B, Zheng J. Biomicrofluidics. 2014 Feb 19;8(1):014108. Doi: 10.1063/1.4864235. Ecollection 2014. Using Microfluidic Chip To Form Brain-Derived Neurotrophic Factor Concentration Gradient For Studying ...
Wan J, Chai H, Yu Z, Ge W, Kang N, Xia W, Che Y. J Exp Clin Cancer Res. 2011 Aug 15;30:77. Doi: 10.1186/1756-9966-30-77. Hif-1?? Effects On Angiogenic Potential In Human Small Cell Lung Carcinoma.
Li K, Zheng S, Xiao X, Wang Q, Zhou Y, Chen L. J Pediatr Surg. 2007 Aug;42(8):1433-8. The Structural Characteristics And Expression Of Neuropeptides In The Esophagus Of Patients With Congenital Esophageal Atresia And Tracheoesophageal Fistula.
Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...
Du Y, Zou Y, Yu W, Shi R, Zhang M, Yang W, Duan J, Deng Y, Wang X, L?? Y. Brain Res. 2013 May 6;1509:79-85. Doi: 10.1016/J.Brainres.2013.03.005. Epub 2013 Mar 18. Expression Pattern Of Sorting Nexin 25 In Temporal Lobe Epilepsy: A Study On Patient...
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Wu J, Yang J, Liu Q, Wu S, Ma H, Cai Y. Biol Trace Elem Res. 2013 Apr;152(1):125-34. Doi: 10.1007/S12011-013-9601-3. Epub 2013 Jan 23. Lanthanum Induced Primary Neuronal Apoptosis Through Mitochondrial Dysfunction Modulated By Ca??
Zhang Z, Zhou X, Zhou X, Xu X, Liao M, Yan L, Lv R, Luo H. Neural Regen Res. 2012 May 5;7(13):971-7. Doi: 10.3969/J.Issn.1673-5374.2012.13.002. Methyl 3,4-Dihydroxybenzoate Promotes Neurite Outgrowth Of Cortical Neurons Cultured In Vitro.
Shao Jl, Wan Xh, Chen Y, Bi C, Chen Hm, Zhong Y, Heng Xh, Qian Jq. J Mol Neurosci. 2011 Mar;43(3):453-60. Doi: 10.1007/S12031-010-9464-4. Epub 2010 Oct 22. H2S Protects Hippocampal Neurons From Anoxia-Reoxygenation Through Camp-Mediated Pi3K/Akt/P...
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Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.
Meng Xt, Chen D, Dong Zy, Liu Jm. Cell Biol Int. 2007 Jul;31(7):691-8. Epub 2007 Jan 19. Enhanced Neural Differentiation Of Neural Stem Cells And Neurite Growth By Amniotic Epithelial Cell Co-Culture.
Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W. J Mol Med (Berl). 2010 Nov;88(11):1181-90. Doi: 10.1007/S00109-010-0659-5. Epub 2010 Aug 10. Isolation Of Cancer Stem Cells From Transformed Human Mesenchymal Stem Cell Line F6.
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.
Xu Yl, Sun Jg, Wu Fb, Xi Ym. J Stem Cells Regen Med. 2011 Oct 30;7(2):69-74. Ecollection 2011. Investigation Of Characteristics Of Feather Follicle Stem Cells And Their Regeneration Potential.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to neuron specific enolase antibody, neuron-specific enolase antibody, nse antibody
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