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Product Info Summary
| SKU: | PA1061 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NSE/ENO2 Antibody Picoband®
View all Enolase 2/Neuron-specific Enolase Antibodies
SKU/Catalog Number
PA1061
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NSE/ENO2 Antibody catalog # PA1061. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NSE/ENO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1061)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NSE, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1061 is reactive to ENO2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
47269 MW
Background of Enolase 2/Neuron-specific Enolase
NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1061 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Positive Control
WB: Rat Brain Tissue
IHC: rat brain tissue
Validation Images & Assay Conditions
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Anti-NSE antibody, PA1061, Western blotting
WB: Rat Brain Tissue Lysate
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Figure 2. IHC analysis of NSE using anti-NSE antibody (PA1061).
NSE was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (PA1061) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ENO2 (Source: Uniprot.org, NCBI)
Gene Name
ENO2
Full Name
Gamma-enolase
Weight
47269 MW
Superfamily
enolase family
Alternative Names
Gamma-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE;ENO2; ENO2 HEL-S-279, NSE enolase 2 gamma-enolase|2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|enolase 2 (gamma, neuronal)|epididymis secretory protein Li 279|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neuronal enriched enolase|neurone-specific enolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENO2, check out the ENO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NSE/ENO2 Antibody Picoband® (PA1061)
Hello CJ!
PA1061 has been cited in 48 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells
Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons
Protective effects of early hypoxic post-conditioning in cultured cortical neurons
HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways:
Stearic acid protects primary cultured cortical neurons against oxidative stress4
Protective effect of Angelica sinensis on cerebral neurons from rat embryos under hypoxia**
Differentiation of neural stem cells into Schwann-like cells in vitro
Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells
Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25%u201335)-induced neuronal injury
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Customer Q&As
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1 Customer Q&As for Anti-NSE/ENO2 Antibody Picoband®
Question
We are currently using anti-NSE/ENO2 antibody PA1061 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-NSE/ENO2 antibody (PA1061) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
