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- Table of Contents
ENO2 antibody (PA1061) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 15 Publications
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IHC analysis of NSE/ENO2 using anti-NSE/ENO2 antibody (PA1061).
NSE/ENO2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NSE/ENO2 Antibody (PA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NSE/ENO2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NSE/ENO2 Antibody (PA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NSE/ENO2 Antibody Picoband®
ENO2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
ENO2 antibody was positively detected by WB in:
Rat Brain Tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ENO2 antibody was positively detected by IHC in:
rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Rat, Human, Mouse
Western blot
0.1-0.5μg/ml
Rat, Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Human
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NSE/ENO2 Antibody Picoband®
View all ENO2 Antibodies
View all ENO2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NSE, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated Molecular Weight
47.3 kDa
Background of ENO2
NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
Cite This Product
Anti-NSE/ENO2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1061)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
87%
Moderate Homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ENO2 antibody
Immunohistochemistry (IHC) protocol for ENO2 antibody
Publications
Application:
Species:
HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways.
Published: 2019-11-06
Journal: CELL TRANSPLANTATION
Application: ICC
Impact Factor: 3.48
Species: Rat
PMID: 31694396
Protective effects of effective ingredients of Danshen (Radix Salviae Miltiorrhizae) and Honghua (Flos Carthami) compatibility after rat hippocampal neurons induced by hypoxia injury
Published: 2018-11-30
Journal: Journal of Traditional Chinese Medicine
Application: IHC
Impact Factor: 0.86
Species: Rat
Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25–35)-induced neuronal injury
Published: 2017-07-01
Journal: Neural Regeneration Research
Application: IHC
Impact Factor: 1.77
Species: Rat
PMID: 28852396
Lanthanum Induced Primary Neuronal Apoptosis Through Mitochondrial Dysfunction Modulated by Ca2+ and Bcl-2 Family
Published: 2013-01-23
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: IHC
Impact Factor: 1.31
Species: Rat
PMID: 23338853
Primary Study for the Therapeutic Dose and Time Window of Picroside II in Treating Cerebral Ischemic Injury in Rats
Published: 2012-02-23
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC
Impact Factor: 2.60
Species: Rat
PMID: 22489110
Nose-to-Brain Transport Pathways of Wheat Germ Agglutinin Conjugated PEG-PLA Nanoparticles
Published: 2011-12-14
Journal: PHARMACEUTICAL RESEARCH
Application: IHC
Impact Factor: 4.46
Species: Rat
PMID: 22167350
HIF-1α effects on angiogenic potential in human small cell lung carcinoma
Published: 2011-08-15
Journal: JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
Application: IHC
Impact Factor: 1.92
Species: Human
PMID: 21843314
Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons
Published: 2011-06-27
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: IHC
Impact Factor: 1.81
Species: Rat
PMID: 21720702
Protective effects of early hypoxic post-conditioning in cultured cortical neurons
Published: 2011-05-11
Journal: BRAIN INJURY
Application: IF
Impact Factor: 1.75
Species: Rat
PMID: 21534738
Differentiation of neural stem cells into Schwann-like cells in vitro
Published: 2010-10-02
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: IF,IHC
Impact Factor: 2.55
Species: Rat
PMID: 20920478
Isolation of cancer stem cells from transformed human mesenchymal stem cell line F6
Published: 2010-08-10
Journal: JOURNAL OF MOLECULAR MEDICINE-JMM
Application: ICC
Impact Factor: 5.00
Species: Human
PMID: 20697686
Induced Multilineage Differentiation of Chicken Embryonic Germ Cells via Embryoid Body Formation
Published: 2010-02-25
Journal: STEM CELLS AND DEVELOPMENT
Application: ICC
Impact Factor: 4.15
Species: Chicken
PMID: 19548770
Protective effect of Angelica sinensis on cerebral neurons from rat embryos under hypoxia**
Published: 2007-09-06
Journal: Neural Regeneration Research
Application: IHC
Impact Factor: 0.00
Species: Rat
Stearic acid protects primary cultured cortical neurons against oxidative stress
Published: 2007-03-01
Journal: ACTA PHARMACOLOGICA SINICA
Application: IHC
Impact Factor: 1.40
Species: Rat
PMID: 17302992
Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells
Published: 2006-11-01
Journal: ONCOLOGY REPORTS
Application: IHC
Impact Factor: 1.57
Species: Human
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We promise Anti-NSE/ENO2 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1061
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026