Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody

Product Name

Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody

View all Enolase 2/Neuron-specific Enolase Antibodies

SKU/Catalog Number

M02930

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody (Catalog # M02930). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02930)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ENO2/1375

Isotype

IgG2b, kappa

Immunogen

A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02930 is reactive to ENO2 in Human, Mouse, Rat

Applications

M02930 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

47.269kDa

Background of Enolase 2/Neuron-specific Enolase

Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.2-0.4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with Anti-NSE gamma Monoclonal Antibody (ENO2/1375).

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Formalin-fixed, paraffin-embedded human Cerebellum stained with Anti-NSE gamma Monoclonal Antibody (ENO2/1375).

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Western Blot of Y79, HeLa and HepG2 cell lysate using Anti-NSE, gamma Monoclonal Antibody (ENO2/1375).

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Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Anti-NSE gamma Mouse Monoclonal Antibody (ENO2/1375).

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Formalin-fixed, paraffin-embedded Rat Heart stained with Anti-NSE gamma Mouse Monoclonal Antibody (ENO2/1375).

Gene/Protein Information For ENO2 (Source: Uniprot.org, NCBI)

Alternative Names

2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; ENO2; enolase 2 (gamma, neuronal); Enolase 2; gamma-Enolase; Neural enolase; neuron specific gamma enolase; neurone-specific enolase; Neuronspecific Enolase; Neuron-specific enolase; NSE ENO2 HEL-S-279, NSE enolase 2 gamma-enolase|2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|enolase 2 (gamma, neuronal)|epididymis secretory protein Li 279|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neuronal enriched enolase|neurone-specific enolase

For more info on ENO2, check out the ENO2 Infographic

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In this infographic, you will see the following information for ENO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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