Product Info Summary
SKU: | A02763-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody
SKU/Catalog Number
A02763-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody catalog # A02763-1. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02763-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein of human CHRNB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02763-1 is reactive to TIA1 in Human, Mouse
Applications
A02763-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42.963kDa
Background of TIA1
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
Cheung P.C.F., Biochem. J. 346:659-669(2000).
Lang T.M., Genomics 70:258-263(2000).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
IP: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of TIA1 using Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody (A02763-1).
ICC staining TIA1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody A02763-1
Western blot analysis of TIA1 on Jurkat cells lysates using anti-TIA1 antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TIA1 (Source: Uniprot.org, NCBI)
Gene Name
TIA1
Full Name
Nucleolysin TIA-1 isoform p40
Weight
42.963kDa
Alternative Names
cytotoxic granule-associated RNA-binding protein; nucleolysin TIA-1 isoform p40; p40-TIA-1 (containing p15-TIA-1); p40-TIA-1; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA1 cytotoxic granule-associated RNA binding protein; TIA1 cytotoxic granule-associated RNA-binding protein; TIA-1 TIA1 ALS26, TIA-1, WDM TIA1 cytotoxic granule associated RNA binding protein nucleolysin TIA-1 isoform p40|nucleolysin TIA-1|T-cell-restricted intracellular antigen-1|p40-TIA-1 (containing p15-TIA-1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TIA1, check out the TIA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TIA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody (A02763-1)
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No publications found for A02763-1
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