Anti-NUF2 Antibody Picoband™

Product Name

Anti-NUF2 Antibody Picoband™

View all Nuf2 Antibodies

SKU/Catalog Number

A03788-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NUF2 Antibody Picoband™ catalog # A03788-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NUF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03788-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NUF2 recombinant protein (Position: M1-Q297).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03788-2 is reactive to Nuf2 in Human

Applications

A03788-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

54.594kDa

Background of Nuf2

Kinetochore protein Nuf2 is a protein that in humans is encoded by the NUF2 gene. This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of NUF2 using anti-NUF2 antibody (A03788-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUF2 antigen affinity purified polyclonal antibody (Catalog # A03788-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUF2 at approximately 54 kDa. The expected band size for NUF2 is at 54 kDa.

Gene/Protein Information For Nuf2 (Source: Uniprot.org, NCBI)

Alternative Names

cancer/testis antigen 106; CDCA1hsNuf2; cell division cycle associated 1; Cell division cycle-associated protein 1; CT106; hNuf2; kinetochore protein Nuf2; NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae); NUF2RhNuf2R

For more info on Nuf2, check out the Nuf2 Infographic

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In this infographic, you will see the following information for Nuf2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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