Click image to see more details
Product Info Summary
| SKU: | PB9162 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TdT/DNTT Antibody Picoband®
SKU/Catalog Number
PB9162
PB0178 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TdT/DNTT Antibody Picoband® catalog # PB9162. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TdT/DNTT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9162)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9162 is reactive to DNTT in Human
Observed Molecular Weight
58 kDa
Calculated molecular weight
58.5 kDa
Background of DNTT
Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template direction catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9162 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Jurkat whole cel
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TdT using anti-TdT antibody (PB9162).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human TDT protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.
Click image to see more details
Western blot analysis of TdT using anti-TdT antibody (PB9162).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cel lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 58 kDa. The expected band size for TdT is at 58 kDa.
Specific Publications For Anti-TdT/DNTT Antibody Picoband® (PB9162)
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Customer Q&As
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16 Customer Q&As for Anti-TdT/DNTT Antibody Picoband®
Question
Is this PB9162 anti-TdT/DNTT antibody reactive to the isotypes of DNTT?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
The immunogen of PB9162 anti-TdT/DNTT antibody is E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-08
Question
We want to test anti-TdT/DNTT antibody PB9162 on human testis for research purposes, then I may be interested in using anti-TdT/DNTT antibody PB9162 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The products we sell, including anti-TdT/DNTT antibody PB9162, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-20
Question
Is a blocking peptide available for product anti-TdT/DNTT antibody (PB9162)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We do provide the blocking peptide for product anti-TdT/DNTT antibody (PB9162). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-27
Question
Would anti-TdT/DNTT antibody PB9162 work for WB with testis?
K. Brown
Verified customer
Asked: 2020-03-19
Answer
According to the expression profile of testis, DNTT is highly expressed in testis. So, it is likely that anti-TdT/DNTT antibody PB9162 will work for WB with testis.
Boster Scientific Support
Answered: 2020-03-19
Question
I see that the anti-TdT/DNTT antibody PB9162 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-06
Question
We are currently using anti-TdT/DNTT antibody PB9162 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?
R. Mitchell
Verified customer
Asked: 2020-02-13
Answer
The anti-TdT/DNTT antibody (PB9162) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-13
Question
My team were well pleased with the WB result of your anti-TdT/DNTT antibody. However we have observed positive staining in bone marrow nucleus. using this antibody. Is that expected? Could you tell me where is DNTT supposed to be expressed?
M. Krishna
Verified customer
Asked: 2020-01-02
Answer
Based on literature, bone marrow does express DNTT. Generally DNTT expresses in nucleus. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2020-01-02
Question
Does PB9162 anti-TdT/DNTT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
As indicated on the product datasheet, PB9162 anti-TdT/DNTT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-TdT/DNTT antibody PB9162. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-13
Question
I am interested in using your anti-TdT/DNTT antibody for dna modification studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
Thanks for your inquiry. This PB9162 anti-TdT/DNTT antibody is validated on jurkat whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-28
Question
I have a question about product PB9162, anti-TdT/DNTT antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9162 anti-TdT/DNTT antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-08
Question
I was wanting to use your anti-TdT/DNTT antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
It shows on the product datasheet, PB9162 anti-TdT/DNTT antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-01
Question
Would anti-TdT/DNTT antibody PB9162 work on zebrafish WB with bone marrow?
S. Anderson
Verified customer
Asked: 2018-03-29
Answer
Our lab technicians have not tested anti-TdT/DNTT antibody PB9162 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TdT/DNTT antibody PB9162 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish bone marrow in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-29
Question
Please see the WB image, lot number and protocol we used for testis using anti-TdT/DNTT antibody PB9162. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-21
Question
We have observed staining in human testis. Are there any suggestions? Is anti-TdT/DNTT antibody supposed to stain testis positively?
E. Carter
Verified customer
Asked: 2016-05-03
Answer
According to literature testis does express DNTT. According to Uniprot.org, DNTT is expressed in bone marrow, thymus, testis, among other tissues. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2016-05-03
Question
Is there a BSA free version of anti-TdT/DNTT antibody PB9162 available?
H. Baker
Verified customer
Asked: 2015-02-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TdT/DNTT antibody PB9162 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-02-17
