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Product Info Summary
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Anti-TdT/DNTT Antibody Picoband™
Boster Bio Anti-TdT/DNTT Antibody Picoband™ catalog # PB9162. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TdT/DNTT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9162)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9162 is reactive to DNTT in Human
PB9162 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TdT using anti-TdT antibody (PB9162).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human TDT protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.
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Figure 2. Western blot analysis of TdT using anti-TdT antibody (PB9162).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cel lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 58 kDa. The expected band size for TdT is at 58 kDa.
Gene/Protein Information For DNTT (Source: Uniprot.Org, NCBI)
DNA polymerase type-X family
deoxynucleotidyltransferase, terminal; DNTT; EC 2.7.7; EC 22.214.171.124; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TdT; TDTDNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; Terminal transferase DNTT TDT DNA nucleotidylexotransferase DNA nucleotidylexotransferase|nucleosidetriphosphate:DNA deoxynucleotidylexotransferase|terminal addition enzyme|terminal deoxynucleotidyltransferase|terminal deoxyribonucleotidyltransferase|terminal transferase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DNTT, check out the DNTT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DNTT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB9162 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang Y, Yu M, Hu X, Han L, Yang K, Ba H, Zhang Z, Yin B, Yang XP, Li Z, Wang J. Cell Death Differ. 2017 Apr;24(4):660-671. doi: 10.1038/cdd.2016.162. Epub 2017 Feb 10. STAT1 mediates transmembrane TNF-alpha-induced formation of death-inducing sig...
Zhang R, Pei H, Ru L, Li H, Liu G. Biomed Rep. 2013 Nov;1(6):895-900. Epub 2013 Sep 6. Bone Morphogenetic Protein 7 Upregulates The Expression Of Nestin And Glial Fibrillary Acidic Protein In Rats With Cerebral Ischemia-Reperfusion Injury.
Xu Bb, Liu Cq, Gao X, Zhang Wq, Wang Sw, Cao Yl. J Asian Nat Prod Res. 2005 Jun;7(3):215-24. Possible Mechanisms Of The Protection Of Ginsenoside Re Against Mptp-Induced Apoptosis In Substantia Nigra Neurons Of Parkinson'S Disease Mouse Model.
Gong Wh, Zheng Wx, Wang J, Chen Sh, Pang B, Hu Xm, Cao Xl. World J Gastroenterol. 2012 Sep 21;18(35):4934-43. Coexistence Of Hyperlipidemia And Acute Cerebral Ischemia/Reperfusion Induces Severe Liver Damage In A Rat Model.
Zeng C, Ke Z, Song Y, Yao Y, Hu X, Zhang M, Li H, Yin J. Exp Mol Pathol. 2013 Aug;95(1):23-31. Doi: 10.1016/J.Yexmp.2013.04.002. Epub 2013 Apr 28. Annexin A3 Is Associated With A Poor Prognosis In Breast Cancer And Participates In The Modulation O...
Xi D, Wang M, Ye H, Luo X, Ning Q. Plos One. 2013 Nov 26;8(11):E82330. Doi: 10.1371/Journal.Pone.0082330. Ecollection 2013. Combined Adenovirus-Mediated Artificial Micrornas Targeting Mfgl2, Mfas, And Mtnfr1 Protect Against Fulminant Hepatic Failu...
Yang Q, Zhang F, Ding Y, Huang J, Chen S, Wu Q, Wang Z, Wang Z, Chen C. Br J Cancer. 2014 Mar 4;110(5):1288-97. Doi: 10.1038/Bjc.2014.1. Epub 2014 Jan 21. Antitumour Activity Of The Recombination Polypeptide Gst-Nt21Mp Is Mediated By Inhibition Of...
Luo T, Xia Z, Ansley Dm, Ouyang J, Granville Dj, Li Y, Xia Zy, Zhou Qs, Liu Xy. Anesth Analg. 2005 Jun;100(6):1653-9. Propofol Dose-Dependently Reduces Tumor Necrosis Factor-Alpha-Induced Human Umbilical Vein Endothelial Cell Apoptosis: Effects On...
Luo T, Xia Z. Anesth Analg. 2006 Jul;103(1):110-6, Table Of Contents. A Small Dose Of Hydrogen Peroxide Enhances Tumor Necrosis Factor-Alpha Toxicity In Inducing Human Vascular Endothelial Cell Apoptosis: Reversal With Propofol.
Tochinai R, Ando M, Suzuki T, Suzuki K, Nagata Y, Hata C, Uchida K, Kobayashi T, Kado S, Kaneko K. Exp Toxicol Pathol. 2013 Sep;65(6):737-43. Doi: 10.1016/J.Etp.2012.09.008. Epub 2012 Nov 4. Histopathological Studies Of Microtubule Disassembling A...
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