Product Info Summary
SKU: | A07925 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-NUMBL Antibody Picoband™
SKU/Catalog Number
A07925
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUMBL Antibody Picoband™ catalog # A07925. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUMBL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07925)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human NUMBL recombinant protein (Position: R336-A537). Human NUMBL shares 91.1% and 89.4% amino acid (aa) sequence identity with mouse and rat NUMBL, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07925 is reactive to NUMBL in Human, Mouse, Rat
Applications
A07925 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
64.891kDa
Background of NUMBL
Numb-like protein is a protein that in humans is encoded by the NUMBL gene. NUMBL [numb (drosophila) homolog-like] is functionally related to NUMB, a protein that plays an essential role in the determination of cell fate for sensory organ and neural development. Polyglutamine repeats in the NUMBL gene have been associated with schizophrenia in two unrelated populations. Alternate names for NUMBL include NBL, CAG3A, CTG3a, NUMBR, TNRC23, and NUMBLIKE.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NUMBL using anti-NUMBL antibody (A07925).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87 MG whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMBL antigen affinity purified polyclonal antibody (Catalog # A07925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMBL at approximately 72 kDa. The expected band size for NUMBL is at 65-70 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of U87 cells using anti-NUMBL antibody (A07925).
Overlay histogram showing U87 cells stained with A07925 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMBL Antibody (A07925, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NUMBL (Source: Uniprot.org, NCBI)
Gene Name
NUMBL
Full Name
Numb-like protein
Weight
64.891kDa
Alternative Names
CAG3A; CTG3a; numb (Drosophila) homolog-like; numb homolog (Drosophila)-like; numb homolog-like; numb-like protein; NUMBLIKE; NUMB-R; Numb-related protein; NUMBRNBL; TNRC23 NUMBL CAG3A, CTG3a, NBL, NUMB-RIKE, NUMBR, TNRC23, NUMBL NUMB like endocytic adaptor protein numb-like protein|numb homolog-like|numb-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NUMBL, check out the NUMBL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NUMBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NUMBL Antibody Picoband™ (A07925)
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No publications found for A07925
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