Click image to see more details
Product Info Summary
| SKU: | A05446 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NVL Antibody
SKU/Catalog Number
A05446
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NVL Antibody catalog # A05446. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NVL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05446)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
8C1B1
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human NVL. at AA rangle: 320-400
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05446 is reactive to NVL in Human, Mouse, Rat
Applications
A05446 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
95.051kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of extracts from rat stomach, 293 cells, using NVL Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NVL (Source: Uniprot.org, NCBI)
Gene Name
NVL
Full Name
Nuclear valosin-containing protein-like
Weight
95.051kDa
Superfamily
AAA ATPase family
Alternative Names
nuclear valosin-containing protein-like; Nuclear VCP-like protein; nuclear VCP-like; NVLp NVL NVL2 nuclear VCP like nuclear valosin-containing protein-like|NVLp
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NVL, check out the NVL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NVL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NVL Antibody (A05446)
Hello CJ!
No publications found for A05446
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-NVL Antibody
Question
My question regarding product A05446, anti-NVL antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05446 anti-NVL antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-NVL antibody A05446 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
The anti-NVL antibody (A05446) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-29
Question
Is this A05446 anti-NVL antibody reactive to the isotypes of NVL?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The immunogen of A05446 anti-NVL antibody is Synthesized peptide derived from the Internal region of human NVL. at AA rangle: 320-400. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-21
Question
Does anti-NVL antibody A05446 work on pig WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
Our lab technicians have not tested anti-NVL antibody A05446 on pig. You can run a BLAST between pig and the immunogen sequence of anti-NVL antibody A05446 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Would A05446 anti-NVL antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
You can see on the product datasheet, A05446 anti-NVL antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NVL antibody A05446. Let me know if you need anything else.
N. Parker
Verified customer
Asked: 2013-12-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-23
Question
I see that the anti-NVL antibody A05446 works with WB, what is the protocol used to produce the result images on the product page?
E. Kulkarni
Verified customer
Asked: 2013-05-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-28
