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Product Info Summary
| SKU: | A01126-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-OAT Antibody (N-term)
SKU/Catalog Number
A01126-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-OAT Antibody (N-term) (Catalog # A01126-1). Tested in WB, IF, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-OAT Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01126-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This OAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01126-1 is reactive to OAT in Human, Mouse, Rat
Calculated molecular weight
48.5 kDa
Background of OAT
OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01126-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Fluorescent image of A549 cell stained with OAT Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with OAT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). OAT immunoreactivity is localized to Mitochondrion significantly.
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All lanes : Anti-OAT Antibody (N-term) at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: human liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 49 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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OAT Antibody (N-term) (Cat. #A01126-1) western blot analysis in 293, 293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane). This demonstrates the OAT antibody detected the OAT protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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OAT Antibody (N-term) (Cat. #A01126-1) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-OAT Antibody (N-term) (A01126-1)
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